Dr Craven - Done

Question 1

What are the units of a unimolecular irreversible reaction rate constant?

Answer 1

per unit time

Question 2

For a unimolecular irreversible reaction what is the rate of change of A

Answer 2

-k[A]

Question 3

For a unimolecular irreversible reaction what is the rate of change of B

Answer 3

k[A]

Question 4

What order is a unimolecular irreversible reaction?

Answer 4

1st order

Question 5

In a unimolecular reversible reaction, what is important about the transition states of the forward and backward reactions?

Answer 5

They are the same

Question 6

Why is one reaction more favourable than the other in a unimolecular reversible reaction?

Answer 6

the energy barrier will be smaller in one direction

Question 7

In a unimolecular reversible reaction what is the rate of A->B?

Answer 7

K1

Question 8

In a unimolecular reversible reaction what is the rate of B->A?

Answer 8

K-1

Question 9

How can a unimolecular reversible reaction be described when K1 = K-1

Answer 9

it is at equilibrium

Question 10

If the reaction A+B->C takes place, what is conditions must A and B meet in in order to make C?

Answer 10

must be in the correct orientation

must have enough energy

Question 11

If the concentration of A is increased in A+B->C, why will the rate increase?

Answer 11

The chance of a successful collision taking place is increased

Question 12

In a Bi-molecular irreversible reaction what is the rate of production of C?

Answer 12

K[A][B]

Question 13

What order is a bi-molecular irreversible reaction?

Answer 13

second

Question 14

What are the units of the rate constant for a bi-molecular irreversible reaction?

Answer 14

concentration per unit time

Question 15

What are the units of KD?

Answer 15

concentration

Question 16

What is the formula for KD in terms of rates?

Answer 16

forward reaction (Kon) / reverse reaction (Koff)

Question 17

What is the formula for KD in terms of concentration?

Answer 17

[P][L]/[PL}

Question 18

What does a larger KD mean?

Answer 18

faster forward rate, faster leaving binding site

slower backward rate, slower into biding site

Question 19

In terms of concentration, what is the amount of fraction bound?

Answer 19

[PL]/[P.tot]

Question 20

If; KD=2uM, [P.tot]=1uM and [L]=8uM, what is the total concentration of ligand added [L.tot]?

Answer 20

[L]/[L]+KD = 8/8+2 = [PL] =0.8
[L]+[PL] = 0.8+8 = [L.tot] = 8.8uM

Question 21

What is the Michaelis Menton equation?

Answer 21

(K.cat x [E.tot] x [S]) / ([S] + Km)

Question 22

What is K.cat?

Answer 22

the rate at which an enzyme will form P if always bound to L

Question 23

What is Km?

Answer 23

Michaelis constant = how well substrate binds to enzyme

Question 24

If Km is lower, what does this describe about the binding of the substrate to the enzyme?

Answer 24

better binding

Question 25

What is a competitive inhibitor?

Answer 25

A molecule which binds to the active site preventing S binding

Question 26

If a competitive inhibitor binds, what is the effect on Km?

Answer 26

It increases

Question 27

If a competitive inhibitor binds, what is the effect on Kcat?

Answer 27

It stays the same