Double Strand Break Repair

Question 1

What causes DS breaks?

Answer 1

Meiosis
DNA replication
Antibody generation
Insertion of retrovirus
Environment

Question 2

Which proteins sense DS breaks?

Answer 2

ATM and ATR

Question 3

End protection is important in which kind of DS break repair?

Answer 3

NHEJ (Non homologous end joining)

Question 4

End resection is important in which kind of DS break repair?

Answer 4

Homologous Recombination

Question 5

When can homologous recombination occur?

Answer 5

During S and G2 phase

Question 6

KU is involved in which type or repair?

Answer 6

NHEJ

Question 7

BRCA1 is involved in which type or repair?

Answer 7

Homologous recombination

Question 8

Artemis is involved in which type or repair? And what does it do?

Answer 8

NHEJ

It has both Endo and exonuclease activity.

Question 9

What is the role of SPO 11?

Answer 9

It initiates double strand breaks in meiosis.

Question 10

How does homologous recombination differ in somatic cells and gametes?

Answer 10

Gametes prefer to use the homolog strand to repair break–> leads to inc. Genetic diversity

Somatic prefer to use sister chromatic to eliminate recombination