DNA Flashcards
What are the 4 factors that contribute to Tm or DNA melting temperature?
Salt
pH
Length of fragment
GC ratio
Which base usually undergoes deamination?
C–>U
Where on a base does alkylation occur?
on nucleophilic nitrogens
DNA is always synthesized in which direction?
5’-3’
Which DNA polymerase is responsible for RNA degradation?
DNA Pol I
In comparison to Eukaryotic cells, prokaryotic cells have ________ origins of replication
Only 1.
Eukaryotes use 100s
Which direction(s) of exonuclease activity does DNA pol I have?
5’-3’ AND 3’-5’
Which DNA polymerase is processive?
DNA pol III
processive means it has a sliding clamp
Why is looping of the lagging strand important?
It allows both strands to be synthesized concurrently
DNA Pol III has which type(s) of proofreading?
3’-5’ ONLY
Which type of DNA repair fixes base pair distortions that distort the DNA?
Nucleotide Excision Repair
If its really bad, cut it all out!
Which type of DNA repair relies on glycosylases?
Base Excision Repair
A defect in Global NER could result in which disease(s)?
Xeraderma Pigmentosum (skin neoplasms)
A defect in transcription-coupled NER could result in which disease(s)?
Cockayne Syndrome (premature aging and impaired development)
Which proteins are involved in recognition during mismatch repair?
MutS (MSH in euk)
MutL (MLH in euk)
