Amino Acids and Proteins

Question 1

How are alpha helices formed?

Answer 1

Backbone interactions 4 AA away (i.e. AA1 binds with AA6)

Ala and Leu–> strong helix formers

Pro and Gly –> helix breakers

Amino acids 1 and 8 stack on top of eachother.

Question 2

Beta Turns are made of which amino acids

Answer 2

Proline in position 2 and glycine in 3.

Question 3

Which amino acids are targeted by the drug Gleevec? What other amino acids contain the same functional group as the target above?

Answer 3

Tyrosine

Gleevec is a tyrosine Kinase inhibitor

Other amino acids that can be phosphorylated are

Serine and Threonine and Tyrosine (all have OH)

Question 4

What does crixivan do?

Answer 4

HIV protease inhibitor

You can get HIV in a creepy van

Question 5

Which amino acids are helix formers?
Breakers?

Answer 5

Ala and Leu=Formers

Gly Pro=Breakers

Question 6

When does O2 begin to bind to hemoglobin? In the Tense state or Relaxed state?

Answer 6

Tense- When you’re tense you need to get some!

Question 7

Give 3 examples of Antibody drugs:

Answer 7

Avastin( bevacizumab) Anti VEGF

Infliximab Anti TNFa

Rituximab Anti CD-20

* Notice all the antibody drugs end in “mab”

Question 8

Hsp40 and Hsp70 (or DNA K and J) are examples of what kind of protein?

Answer 8

Chaparone- they help proteins cross membranes in unfolded states

Question 9

What process can tell us the N-terminal sequence?

Answer 9

Edman Degredation

Question 10

Which is used up in a reaction, a coenzyme or cofactor?

Answer 10

Coenzymes

Question 11

On a lineweaver-burk plot, what does the y-intercept represent?

X-intercept?

Answer 11

Y=1/Vmax

X=-1/Km