Donor Testing Flashcards
True or False: The integral donor tubing segments shall be separable from the container without breaking the sterility of the container
True
Which method is the strongest to detect weak subgroups?
ABO testing
Which method is able to detect Weak D?
Weak D is called what?
- Rh testing
- Rh positive
Which method give a positive antibody in plasma that does not defer the donor?
Antibody Screen testing
Is antibody Screen performed at every donation?
No
Which method is done at random, and donors are tested by the donor center in order to locate rare antigens?
Phenotype testing
True or False: Some donations are labeled without testing if 2 previous tests from the donor center match records.
True
What tests are required in order to keep the blood supply safe?
- Blood Type
- Antibody Screen
- Infectious Disease Testing
- Quality Control
What test is required to be done in all blood supply?
Blood Type
- ABO Group: Forward and reverse
- D Phenotype: weak D testing required on all Rh negative red cells
When do we do Antibody screen in blood supply?
It is performed on first time donors who have been transfused or pregnant.
Which infectious diseases are tested?
- Hepatits B: HBV DNA, HBsAg, anti-HBc
- Hepatits C: anti-HCV, HCV RNA
- HIV: anti-HIV -1/2, HIV-I RNA, anti-HTLV-I/II
- West Nile virus RNA
- Syphilis serology
- Antibodies to T.cruzi
- Babesia
Quality Control is done to keep the blood supply safe and is required for:
- platelet bacterial testing
- WBC Count on Leukoreduced units
What are the 3 Infectious Diseases that are tested and are permanent deferral?
- Hepatitis B
- Hepatitis C
- HIV
What is the 1 infectious disease that is tested and is a indefinite deferral?
Antibodies to T.cruzi
What are the 3 infectious disease that are tested and are temporary deferrals?
- West Nile Virus
- Syphilis
- Babesia
What is the window period and deferrals associated with FDA required testing for Treponema palladium (Syphilis)?
- Window period: 2-3 weeks
- Deferral: 12 months post treatment
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Treponemes are fragile and sensitive to cold. Risk of transmission would decrease in cold storage of products. More likely to transfuse from fresh blood or platelets.
What is the window period and deferrals associated with Hepatitis B Virus (HBV)?
- Window period: 3-4 weeks
- Deferral: Permanent
True or False: Before testing of all donor products Transfusion associated Hepatitis infection = 30%
True.
- Reason all volunteer blood donor program was mandated by the FDA; it Reduced hepatitis infection by 70%
After _______ testing, Hepatitis infection was still 10% (nonA non B)
HBsAg (Hepatits B surface Antigen)
- 1st gen Radioimmuno assay (toxic to workers)
- 2nd gen EIA
- 3rd gen NAT introduced 2009
_____ was introduced after HBsAg, and HBV DNA testing.
Anti-HBc (hepatitis B core total antibody)
- Total= chronic; Igm= Acute
- Nonspecific indicator of HIV and HepB infection, discounted in 1996.
What is the different between Anti-HBc and anti-HBs?
- Anti-HBc: hepatitis B core total antibody
- anti-HBs:Hepatitis B surface antibody (test to check for immunity)
True or False: HBsAg was the first viral marker sequenced
True
Confirmatory test is __________ of HBsAg
Neutralization
What is the window period and deferrals associated with Human Immunodeficiency Virus (HIV 1 & 2)?
- Window period - ELISA: 56 days. NAT: 7-10 days
- Deferral: Permanent
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Combo test of Mini pool testing and repeated antibody differentiation tests by ELISA
