Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Micro/Immuno Part 2 > DNA Virus 1 > Flashcards

DNA Virus 1 Flashcards

1
Q

key concepts of DNA viruses

A
  • transcription and replication in nucleus (except pox)
  • host RNA pol II transcribes mRNA (except pox)
  • viral or hose DNA pol replicates genome
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

parvovirus

A

-BK virus

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

papova

A

warts

cancer

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

adeno

A

colds

keratitis

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

hepadna

A

hep B

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

herpes

A

cold sores, etc

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

pox

A

smallpox

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

DNA genomes

A
  • small circular DNA

- ejected various ways into the nucleus-see slide

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

steps in DNA virus replication

A
  1. recognition
  2. attachment
  3. entry
  4. uncoating
  5. transcription of mRNA-Host RNA pol
  6. protein synthesis
  7. replication of the genome- host or viral DNA pol
  8. assembly of virions
  9. egress
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

use of host RNA pol for mRNA transcription

A
  • DNA viruses use the host RNA pol
  • RNA pol II and TF (x,y) recognize viral promoters
  • X and Y are proteins that enhance viral transcription
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

TFs

A
  • both cellular and viral factors regulate transcription by host RNA pol
  • viral TFs are important virulence factors and are probably essential
  • need cells to recognize viral TFs and promoters in order to transcribe them
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

genome synthesis-host DNA pol

A
  • some virus genomes are recognized by the host DNA pol complex
  • these viruses do not encode their own polymerase-small genomes
  • parvovirus-adeno associated virus 2
  • need to be in dividing cells
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

genome synthesis-viral DNA pol

A
  • large DNA viruses encode their own pol and accessory proteins
  • herpes, adeno
  • don’t have to be in a replicating cell
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

origins of diversity in DNA viruses

A
  • recombination between and within genomes
  • DNA pol have higher fidelity than RDRP
  • mutations about 1/ 1 mil- like us
  • genetically more stable than RNA viruses
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

adenovirus diseases

A
  • resp-bad cold/fever, pharyngoconjunctival fever, laryngitis and cough, pneumonia
  • other sites-acute hemorrhagic cystitis, epidemic keratoconjunctivitis, gastroenteritis
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

adeno epidemeology

A
  • aerosol, fecal oral, objects
  • poorly chlorinated pools
  • children
  • military recruits
  • daycare centers, swimming clubs, boot camp
17
Q

adenovirus life cycle

A
  • adeno fiber protein binds to cell receptor
  • entry by endo
  • endosome acidification causes fibers to lyse the vesicle
  • capsid traffics to nucleus and DNA genome uncoats through nuclear pore
  • genome replicates in nucleus
  • host RNA pol II makes mRNA
  • immediate early, early, late
  • genome replication by viral DNA pol
  • capsid assembly in nucleus
  • virions egress by lysis
18
Q

gene expression phases in adeno

A
  • immediate early for EIA
  • helps make more mRNA
  • early for DNA pol, which replicates genome
  • late for structural proteins- for assembly
19
Q

adenovirus diagnosis and treatment

A
  • match the diagnostic test to the patient sample
  • culture, antigen detection, PCR, serology
  • treat immunocompromised patients with cidofovir-dire cases and nephrotoxic
20
Q

adeno prevention

A
  • vaccine
  • used only for military personnel
  • reinstated for use in late 2011
  • incidence of Ad4 infections plummeted
21
Q

HPV diseases

A
  • common
  • epithelial disease-common, plantar, genital warts
  • malignancies-head and neck cancer, cervical cancer, penile cancer
22
Q

HPV tissue tropism

A
  • differentiated epithelial cells

- infects basal cells, limited amplification and maintenance replication until to top layer, then productive replication

23
Q

HPV infection

A
  • cut
  • productive life cycle for 2-3 weeks,grows up with skin
  • growing cells express E7/E6, virus sheds
  • lack of immune clearance
  • skin becomes overgrown with HPV infected cells- oncogene activation
  • causes invasive carcinoma
24
Q

HPV DNA replication

A
  • host RNA pol transcribes viral mRNA
  • host DNA pol synthesizes viral genomes
  • viral factors E6 and E7 are oncogenes
25
Q

HPV diagnosis and treatment

A
  • diagnosis for genital HPV is recommended-DNA tests for type, important to screen for 13 high risk types linked to cancer
  • treat warts by excision- freezing, chemical ablation, coposcopy
  • treat malignancies with oncotherapy
26
Q

HPV vaccine

A
  • gardasil-quadrivalent for 6,11,16,18
  • ceravix is 16,18
  • both are virus like particles-empty capsids- makes antibodies to L proteins in genome

Micro/Immuno Part 2 (17 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2025 Bold Learning Solutions. Terms and Conditions