DNA technology

Question 1

restriction endonuclease

Answer 1

enzyme produced by bacteria

Question 2

plasmid

Answer 2

a small circular self replicating dna molecule that inhabits a bacterium

Question 3

recombinant dna

Answer 3

genes from 2 different sources are linked

Question 4

inverted repeat

Answer 4

sequence of nucleotides followed by downstream by reverse compliment

Question 5

recognition sequence

Answer 5

site of dna binding protein motif that exhibits binding

Question 6

electrophoresis

Answer 6

separates restriction fragments

Question 7

transformation

Answer 7

induced or spontaneous change of one element into another

Question 8

regulatory gene

Answer 8

involved in controlling expression of one or ore genes

Question 9

trp operon

Answer 9

consists of a promoter and 5 adjacent structural genes. binds to repressor protein blocks transcription

Question 10

lac operon

Answer 10

binds to repressor allowing gene transcription

Question 11

6 steps used in production of recomb dna

Answer 11

isolation, insertion, introduce, cloning, identity, grow cels