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Biology block 4 > DNA technology > Flashcards

DNA technology Flashcards

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AP® Biology flashcards Biology 101 flashcards
1
Q

colne made possible by the discovery of ?

A

restraction enzymes
plasmid DNA vectors

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2
Q

DNA cutting enzymes

A

restraction enzyme

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3
Q

circular form of self-replicating DNA

A

plasmid DNA vectors

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4
Q

restraction enzyme is primalry found in ?

A

bacteria

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5
Q

what is the function of rastraction enzyme ?

A

cut DNA by cleaving the phosphodiester bond that joins adjacent nucleotide
bind to ;recognize and cut DNA within specific sequences of bases in restraction site

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6
Q

why there are 4 or 6 cutters ?

A

becuse they recognize restraction site with a sequences of 4 or 6 nucleotides

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7
Q

how the fragments with cohesive ends are produced?

A

by digastion of DNA by EcoRI

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8
Q

what blocks the DNA clevage ?

A

methylation of the EcoRI restraction

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9
Q

restraction of single strand ends ///
double strands//

A

sticky-cohasive

blunt ends

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10
Q

which end is prefered for cloing DNA ?

A

cohasive end because it easily joind togather
by forming weak H bound

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11
Q

what is the vector in cloing DNA ?

A

plasmid DNA

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12
Q

RECOMBINANT

A

transformation
by adding forgin dna
electroportion making tiny holes that allow the DNA to enter
selection
1 antibiotics
2 blue-white selection

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13
Q

if funcional lacZ is present

A

blue colony

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14
Q

if funcional lacZ is not ?

A

white colony

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15
Q

library screening with DNA probe to identify a cloned gene interest

A

colony hybridaztion

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16
Q

library screening rarely result in

A

the coloing of the full length

17
Q

hybridization filter

A

nylon then it washed
filter is incubeted with probe

18
Q

advantage of cDNA libraries ?

A

mRNA is isolated
introns are not cloned

19
Q

making double strnad of DNA

A

1 complementary single strand DNA is is catalyzed by reverse transcriptase
secnod one is synthesized by DNA pol

20
Q

mRNA is degraded //?

A

either with an enzyme or alklaine sloution

21
Q

disadvantage of genomic libraries

A

introns are cloned in addtion to exons
time consuming

22
Q

searching for gene of interst is difficult with genomec libraries?

A

many organisms have very large genome

Biology block 4 (5 decks)

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