DNA sequencing Flashcards

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1
Q

uses of DNA sequencing

A

disease management by identifying a faulty gene
predicting the amino acid sequence by analysing base pairs

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2
Q

the materials needed for the sanger technique

A

A DNA polymerase enzyme
a primer (ss DNA that acts a starter for polymerase)
the 4 DNA nucleotides (ATCG)
termitary bases
the template DNA to be sequenced

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3
Q

The methodology steps

A

1) denature DNA with heat
2) make multiple copies of a segment
3) attach a primer
4) add polymerase, bases and terminator bases to a solution
5) grow complementary chains until terminator base
6) denature the growth chains
7) electrophorese the solution

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4
Q

how data is recorded

A

fragments run through a capillary gel electrophoresis
short fragments move quicker through pores of gel than long fragments
as each fragment crosses the “finish line” it is illuminated by laser so attached dye is detected
data recorded by detector as a series of peaks which correspond to DNA sequence

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