7.1 electrophoresis and blotting Flashcards
1
Q
southern blotting
A
the process of transferring DNA fragments to a nylon membrane
2
Q
how fragments are made for gel electrophoresis
A
using restriction endonuclease enzymes to cut DNA at restriction sites in the intron sequence
3
Q
steps of gel electrophoresis
A
fragments added to well of agarose gel
current passe through gel so negative DNA moves down gel to positive electrode
smaller fragments move faster so travel further
4
Q
why is southern blotting done
A
to make the bonding pattern more stable
5
Q
the two possible staining methods
A
staining with dye
probes
6
Q
how staining with dye works
A
use ethidium bromide
fluoresces under UV light
older method
7
Q
Charge on DNA
A
Negative
