DNA replication 4

Question 1

Why is DNA replicated

Answer 1

Question 2

What are the different DNA replication scenarios

Answer 2

Question 3

How was semi conservative replication validated

Answer 3

Question 4

What is the expected results of meselson and stahls experiment if conservative replication is how DNA is replicated

Answer 4

Question 5

What is the expected results of meselson and stahls experiment if semi conservative replication is how DNA is replicated

Answer 5

Question 6

What is the expected results of meselson and stahls experiment if dispersive replication is how DNA is replicated

Answer 6

Strands would have a mix of 14N & 15N so would have appeared as an intermediate density

Question 7

What were the actual results of meselson and stahls experiment

Answer 7

Question 8

What structure can form when bacterial chromosomes are replicated

Answer 8

Question 9

What is meant by DNA replication being bidirectional

Answer 9

Question 10

What is the role of DNA polymerase in replication

Answer 10

Question 11

How is a deoxyribonucleoside triphosphate (dNTP) added to the growing dna strand

Answer 11

Question 12

What do the 2 major DNA polymerases do

Answer 12

Question 13

How is DNA unwound

Answer 13

Question 14

How does DNA helicase work

Answer 14

Question 15

How are the separated DNA strands prevented from re-annealing

Answer 15

Question 16

What is the role of SSBs

Answer 16

Question 17

How do SSBs work

Answer 17

Question 18

What is one issue with DNA replication

Answer 18

Question 19

What would tightening of the DNA helix cause

Answer 19

Question 20

What are topoisomerases and what do they do

Answer 20

Question 21

What do RNA primers do in DNA replication

Answer 21

An RNA primer is a short segment of single-stranded RNA used as a binding site for DNA polymerase to initiate DNA replication. DNA polymerase is only able to add nucleotides to an existing nucleotide chain; it cannot “start from scratch,” and therefore needs the RNA primer to start the process.

Question 22

What is the mechanism of topoisomerase 1

Answer 22

Question 23

How is chain synthesis initiated in DNA replication ( eukaryotic )

Answer 23

Question 24

What do replication forks allow

Answer 24

Question 25

How is the leading and lagging strand synthesised IN DNA replication

Answer 25

Question 26

What is needed for semi discontinuous replication of the lagging strand

Answer 26

Question 27

How many leading and lagging strands are there

Answer 27

Question 28

What is the origin of replication

Answer 28

Question 29

How many origins of replication can you find in eukaryotes

Answer 29

Question 30

What is the role of DNA polymerase I

Answer 30

Question 31

What is DNA polymerase made up of

Answer 31

Question 32

Compare the characteristics of the 3 classes of DNA polymerases

Answer 32

Question 33

What is a sliding clamp on a DNA polymerase

Answer 33

Question 34

What is the structure of DNA polymerase III

Answer 34

Question 35

What is a ß sliding camp

Answer 35

Question 36

What is the accuracy of DNA polymerases

Answer 36

Question 37

Explain second mechanism for polymerase proof reading

Answer 37

Question 38

In more detail explain the process of polymerase proofreading

Answer 38

Question 39

Name all of the proteins involved in DNA replication

Answer 39

Question 40

What is the replisome

Answer 40

Question 41

What are the first two steps of DNA replication

Answer 41

Question 42

What is the 3rd step of DNA replication

Answer 42

Question 43

What is the fourth and fifth step of DNAreplication

Answer 43

Question 44

How is the synthesis of the lagging strand synthesised

Answer 44

Question 45

What does DNA ligase do in replication

Answer 45