DNA replication 4 Flashcards
Why is DNA replicated
What are the different DNA replication scenarios
How was semi conservative replication validated
What is the expected results of meselson and stahls experiment if conservative replication is how DNA is replicated
What is the expected results of meselson and stahls experiment if semi conservative replication is how DNA is replicated
What is the expected results of meselson and stahls experiment if dispersive replication is how DNA is replicated
Strands would have a mix of 14N & 15N so would have appeared as an intermediate density
What were the actual results of meselson and stahls experiment
What structure can form when bacterial chromosomes are replicated
What is meant by DNA replication being bidirectional
What is the role of DNA polymerase in replication
How is a deoxyribonucleoside triphosphate (dNTP) added to the growing dna strand
What do the 2 major DNA polymerases do
How is DNA unwound
How does DNA helicase work
How are the separated DNA strands prevented from re-annealing
What is the role of SSBs
How do SSBs work
What is one issue with DNA replication
What would tightening of the DNA helix cause
What are topoisomerases and what do they do
What do RNA primers do in DNA replication
An RNA primer is a short segment of single-stranded RNA used as a binding site for DNA polymerase to initiate DNA replication. DNA polymerase is only able to add nucleotides to an existing nucleotide chain; it cannot “start from scratch,” and therefore needs the RNA primer to start the process.
What is the mechanism of topoisomerase 1
How is chain synthesis initiated in DNA replication ( eukaryotic )
What do replication forks allow
How is the leading and lagging strand synthesised IN DNA replication
What is needed for semi discontinuous replication of the lagging strand
How many leading and lagging strands are there
What is the origin of replication
How many origins of replication can you find in eukaryotes
What is the role of DNA polymerase I
What is DNA polymerase made up of
Compare the characteristics of the 3 classes of DNA polymerases
What is a sliding clamp on a DNA polymerase
What is the structure of DNA polymerase III
What is a ß sliding camp
What is the accuracy of DNA polymerases
Explain second mechanism for polymerase proof reading
In more detail explain the process of polymerase proofreading
Name all of the proteins involved in DNA replication
What is the replisome
What are the first two steps of DNA replication
What is the 3rd step of DNA replication
What is the fourth and fifth step of DNAreplication
How is the synthesis of the lagging strand synthesised
What does DNA ligase do in replication
