DNA replication Flashcards

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1
Q

what’s the role of the DNA helicase?

A

unwinds DNA and breaks hydrogen bonds between base pairs

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2
Q

what is the template strand?

A

a single stranded DNA the has been unwound and separated from the other strand

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3
Q

where are the free DNA nucleotides found and what do they do?

A

found in the nucleus and attach to complementary bases on the template strand

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4
Q

what is DNA polymerase?

A

the enzyme involved in the condensation reaction joining adjacent nucleotides together, reforming the double stranded DNA

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5
Q

does the strand run parallel or anti parallel?

A

anti parallel

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6
Q

what are the two ends of the strand and how is DNA read from it?

A

5’ end and 3’ end - DNA is always read from 5’ to 3’

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7
Q

what is stage 1 of DNA replication?

A

DNA helicase unwinds double stranded DNA breaking hydrogen bonds, leaving two template strands

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8
Q

what is stage 2 of DNA replication?

A

DNA polymerase binds at the 5’ end of the template strand of DNA

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9
Q

what is stage 3 of DNA replication?

A

free DNA nucleotides are joined together by DNA polymerase in a condensation reaction, forming phosphodiester bonds

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10
Q

what is stage 4 of DNA replication?

A

the leading strand can be copied from start to finish in one entire section

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11
Q

what is stage 5 of DNA replication?

A

the lagging strand has to be copied in sections as the double stranded DNA is being unwound

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