DNA Replication

Question 1

DNA Primase

Answer 1

Synthesizes primer

Question 2

SSB Proteins

Answer 2

Single-strand binding proteins
Prevents uncoiled strands from recoiling

Question 2

Primer

Answer 2

Primase complements the primer sequence in order to start synthesizing the original DNA template strand

Question 3

DNA Polymerase

Answer 3

Synthesizes from 5’ to 3’

Question 4

DNA Helicase

Answer 4

Unwind the DNA strands

Question 5

DNA Topoisomerase

Answer 5

Prevents supercoiling, relaxes tension in replication fork

Question 6

Ligase

Answer 6

Complements the Okazaki fragments

Question 7

What does semiconservative replication mean?

Answer 7

Parent DNA uncoils at the replication fork
One of them will be a template for the complementary strand

Question 8

Coding strand

Answer 8

Thrown away strand

Question 9

Non-coding strand

Answer 9

Template, complemented strand

Question 10

Prokaryotic replication
Where?
Frequency?
Okazaki?
What is the outcome?
Origin of replication?

Answer 10

Cytoplasm
Continuous
Large
Double-stranded circular
Single origin of replication

Question 11

Eukaryotic replication
Where?
Frequency?
Okazaki?
What is the outcome?
Origin of replication?

Answer 11

Nucleus
S phase
small
Double-stranded linear
Multiple origins of replication