DNA Replication

Question 1

chromosomes can maintain its structure due to

Answer 1

the special protein in its core (HISTONE)

Question 2

What do RNA Primers do?

Answer 2

are a starting point for DNA strand elongation by DNA polymerase

Question 3

what direction does DNA Polymerase go

Answer 3

5’ to 3’

Question 4

semiconservative

Answer 4

each DNA strand makes a copy of its complimentary base pair

Question 5

which enzyme relieves tension from the unwinding of supercoils?

Answer 5

DNA Gyrase/ topoisomerase

Question 6

which enzyme joins the phosphodiester bonds between Okazaki fragments?

Answer 6

Ligase

Question 7

Chargaff’s rule

Answer 7

paring of bases (A=T G=_C)

Question 8

DNA Polymerase Functions

Answer 8

• insert new bases during elongation, using the parental strand as a template
• proof reads new strands (and corrects them)
• can differentiate between the parent and new strand
• Removes primers and replaces them with DNA

Question 9

DNA Helicase function

Answer 9

to break the H+ Bonds between 2 strands of DNA molecules

Question 10

SSB Meaning and function

Answer 10

Single Stranded binding proteins
Blocks the H+ bonds in between two parental strands from reforming (RE-ANNEALING)

Question 11

RNA Primase function

Answer 11

Reads DNA and forms a complimentary primer

Question 12

When does replication happen?

Answer 12

S-phase

Question 13

When is DNA condensed?

Answer 13

beginning of M phase

Question 14

what structure holds two sister chromatids

Answer 14

centromere