DNA replication

Question 1

helicase

Answer 1

unwinds parental and double helix at replication forks

Question 2

single-strand binding protein

Answer 2

binds to stabilize single-stranded DNA until it is used as a template

Question 3

topoisomerase

Answer 3

relives overwhelming strain ahead of replication forks by breaking, swiveling and rejoining DNA strands

Question 4

primase

Answer 4

synthesis an RNA primer at 5’ end leaving strand and at 5’ end of each okazaki fragment of lagging strand

Question 5

DNA pol III

Answer 5

using parental DNA as a template, synthesize new DNA strand by adding nucelotides to an RNA primer or a pre-existing DNA strand

Question 6

DNA pol I

Answer 6

removes RNA nucleotides of primer from 5’ end and replaces them with DNA nucleotides added to 3’ end of adjacent fragment

Question 7

DNA ligase

Answer 7

joins Okazaki fragments of lagging strand: on leading strand, join 3’ end of DNA that replaces primer to rest of leading strand DNA