DNA Replication Flashcards

1
Q

what is a nucleoside?

A

base + sugar

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2
Q

what is a nucleotide?

A

nucleoside + phosphate

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3
Q

what are purines and what are examples purines?

A

purines are two rings
examples: adenine and guanine

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4
Q

what are pyramidines and what are examples of water pyramidines?

A

pyramidines are one ring structures
examples: uracil, thymine and cytosine

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5
Q

what type of bond is between 3’ OH group and 5’ triphosphate?

A

phosphodiester bonds

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6
Q

what are the base pairing rules and what bonds do they have ?

A

A - T ( two hydrogen bonds)
C - G( three hydrogen bonds)

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7
Q

what direction does DNA replicate in?

A

5’ prime to 3’ prime direction

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8
Q

what catalysis DNA replication?

A

DNA polymerase

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9
Q

what kind of primer is required for DNA replication?

A

RNA primer

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10
Q

how is the lagging strand replicated?

A

in short fragments called okazaki fragments

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11
Q

what strand always has a free 3’ prime end?

A

the leading strand

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12
Q

what unwinds DNA?

A

helicase proteins

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13
Q

what are the three main classes of RNA?

A

rRNA
tRNA
mRNA

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14
Q

what’s rRNA?

A

ribesomalRNA
combines with proteins to form ribesomes where protein synthesis takes place

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15
Q

what is tRNA?

A

transferRNA
carries amino acid to be incorporated into protein
anti codons consist in three nucleotides

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16
Q

what is mRNA?

A

messengerRNA
carries genetic information for protein synthesis

17
Q

what is the structure of RNA?

A

single stranded
contains a stem loop

18
Q

what type of complex are RNA polymerase?

A

multi-subunit complexes