DNA Replication Flashcards
1
Q
DNA Gyrase
A
- Enzyme that helps unwind DNA by reducing the tension in the double helix
2
Q
DNA Helicase
A
- Break the H bonds of the DNA stands and unwinds it
3
Q
ssB’s
A
- Protiens that make sure the strands don’t reanneal
4
Q
RNA Primase
A
- Enzyme that attaches the RNA primer to the exposed 3’ end of the replication fork
5
Q
RNA Primer
A
- 10 to 60 base strands of RNA that serves as a starting point for DNA synthesis
6
Q
DNA polymerase III
A
- Enzyme that adds complimentary bases in the 5’ to 3’ direction
- Energy released by removing 2 phosphate allows for bonding
- The leading strand is copied continuously towards the replication fork
7
Q
DNA polymerase I
A
- Removes the RNA primer and replaces them with the appropriate DNA bases
8
Q
Okazaki fragments
A
- DNA polymerase III only moves in the 5’ to 3’ direction so the lagging strand is built in segments called Okazaki fragments
9
Q
DNA Ligase
A
- Attaches Okazaki fragments by creating phosphodiester bonds
10
Q
DNA polymerase II
A
- Proofreads DNA and fixes the incorrect nucleotides
- When proofread is finished, termination occurs when the 2 DNA strands separate from
