DNA POLYMORPHISM

Question 1

refers to the presence of genetic variations or differences in the DNA sequences among individuals of a species or within a population

Answer 1

DNA POLYMORPHISM

Question 2

Why do polymorphism exist

Answer 2

Recombination
Mutation
Gene flow and drift
De novo variation

Question 3

Types of dna polymorphism

Answer 3

Single nucleotide polymorphism
Insertions and deletions
Tandem repeats

Question 4

Point mutations involving a single nucleotide

Answer 4

Single nucleotide polymorphism

Question 5

Single nucleotide polymorphism application

Answer 5

genetic studies, association studies, & personalized medicine

Question 6

How many basepairs for 1snp

Answer 6

300-1000 bp

Question 7

Example of snp

Answer 7

rs4680 SNP or the COMT Val158Met polymorphism

Question 8

Variations involving the insertion or deletion of nucleotide sequences

Answer 8

Insertion and Deletion (Indels)

Question 9

can cause frameshift mutations and influence gene function

Answer 9

Insertion and deletion (indels)

Question 10

Example of insertion and deletion (indels)

Answer 10

Huntington disease, a neurodegenerative disorder caused by expansion of CAG repeats

Question 11

Variations in the number of repeated DNA sequences within a specific genomic region

Answer 11

Tandem repeats

Question 12

Applications of tandem repeats

Answer 12

Forensic DNA analysis, paternity testing, and population studies.

Question 13

Types of tandem repeats

Answer 13

Variable number tandem repeat
Short tandem repeat

Question 14

repeats of 10-50 base sequences in tandem; found in minisatellite DNA regions

Answer 14

Variable number tandem repeats

Question 15

repeats of 1-10 base sequences in tandem; found in microsatellite DNA regions

Answer 15

Short tandem repeats

Question 16

Techniques for Detecting DNA Polymorphism

Answer 16

DNA Sequencing
PCR
SOUTHERN BLOT

Question 17

How does southern blot work

Answer 17

1. Dna digestion
2. Gel electrophoresis
3. Blotting and Baking/UV
4. Hybridization
5. Detection and Analysis

Question 18

a method for sizing fragments of DNA that are detected using a complementary DNA probe. It can also be used to determine DNA methylation status.

Answer 18

Southern Blot

Question 19

Importance of dna polymorphism

Answer 19

Evolutionary adaptation
Disease association
Genetic diversity

Question 20

Directly reads DNA sequences for detecting polymorphisms.

Answer 20

Dna sequencing

Question 21

Amplifies specific DNA regions for analysis

Answer 21

PCR

Question 22

Identifies differences of DNA sequence between alleles of a gene

Answer 22

Southern Blot