DNA Flashcards
Detect, Amplify and Quantify DNA or RNA sequences
DNA
NAAT only need what specific target
DNA
- contains the genetic information
in the form of nucleotides
which ultimately affects the anatomy & physiology of individuals.
DNA
Storage forms of dna
Nucleotides
Dna+ protein
Chromatin
Bound to histone
Chromatin
Structural unit of chromatin
Nucleosomes
Regulates the expression of the genes
Non-protein
Condensed form of the chromatin
Chromosome
Chromosome is seen during
Meosis
Four types of nucleotides
Deoxyadenosine monophosphate
Deoxyguanosine monophosphate
Deoxytidine monophosphate / Deoxy thymidine
Deoxycytidine monophosphate
RNA : uracil - Uridine monophosphate
Contains 1 carbon ring
Pyrimidune
2 ketones
Thymine
Cytosine
Ketone and anime
A type of covalent bond that connects the sugar-phosphate backbone of DNA nucleoside
Phosphodiester
A type of covalent bond that connects the sugar and nitrogen base of the DNA Molecule
N-glycosidic
Connects the nitrogen base pairs
Weak hydrogen
Essentially hold the 2 DNA strands
Weak hydrogen
A-T how many hydrogen
2
C-G how many hydrogen
3H
right - handed helix, shorter but wider ; seen among DNA-RNA HYBRIDS
A-form
Mostly found during transcription
A-form
Most common in human cells, right handed helix, longer but narrower
B-form
Rare; left-handed helix, seen with alternating purine pyrimidine bp
Z-form
Major
12 angstroms wide
Minor
6 angstroms
3’
Deoxyribose
5’
Phosphate
Refers to the syntesis of a new DNA strand. It happens in the S phase. Takes 8-10 hours to complete the replication phase. Around 50-300 m bp are replicated per chromosome in a peed of 1000 bp/ sec/ replication fork
Dna Replication
Steps in DNA Replication
- Initiation
- Elongation
- Termination
Main polynerizer for prokaryotes
III.
Contains 2 carbon ring
Purines
Purines consists of
Adenine
Guanine