DNA, genes and Protein synthesis Ch.8 Flashcards
Definition of Gene
A section if DNA on a chromosome coding for one or more polypeptides.
Why is the genetic code said to be universal?
All organisms use the same genetic code
Locus
The position on a chromosome where a gene is found
Universal
All organisms have the same genetic code
Genome
All the genes contained within the cell (includes mitochondria and chloroplast)
Degenerate
An amino acid may be coded for by more than one codon
Redundant
Same as degenerate, when an amino acid may be coded for by more than one codon
(Triplet) Codon
Three nucleotides that correspond to a specific amino acid or stop signal
Allele
An alternative form of a gene
Histone
Protein around what DNA is wrapped around. In this form chromosomes are called chromatin.
Homologous
A pair of chromosomes where one is provided by the father and one by the mother.
Proteome
All the proteins that are coded for by the genome
Intron
Sequence of non-coding DNA
Exon
Sequence of coding DNA
Chromosome
Strand of DNA encoded with genes
Non-overlapping
Each base is only read once in a sequential order.
Diploid
A cell containing two sets of chromosomes one from mother and one from father.
Prokaryotes DNA structure?
Short, circular and not associated with proteins.
Eukaryotes DNA structure?
long, linear and associated with proteins, called histones. Form chromosomes
Whats the structure of DNA in the mitochondria and chloroplast?
Like prokaryotes, short, circular and not
associated with protein.
What’s the difference between RNA and DNA?
DNA is double stranded, and has Thymine, and has ribose sugar.
What’s the structure of mRNA?
A single long stand in a single helix.
What’s the structure of tRNA?
Folded single stranded chain, has anticodon.
Which has hydrogen bond? DNA, tRNA, mRNA
DNA and tRNA
Put in order of most stable to least stable. tRNA, mRNA and DNA
DNA, tRNA then mRNA
What is transcription?
Producing mRNA from tRNA
How is mRNA made?
- DNA helicase breaks the H bonds b/n bases and the DNA unwinds and unzips.
- One of the two stands act as a template strand.
- Free nucleotide pair with complementary bases on template strand.
- RNA polymerase joins the nucleotides forming pre-mRNA
- Pre-mRNA is spliced and introns are removed.
- mRNA leaves via nuclear pore
What happens in splicing?
Introns (non-coding DNA is removed)
Does splicing occur in prokaryotes?
No
What is translation?
The production of polypeptides from mRNA
Explain the process of translation
- Ribosome attaches to the starting codon of the mRNA,
- A tRNA with complimentary anticodon sequence to mRNA moves to ribosomes and pairs with the mRNA.
- The tRNA carries a specific amino acid
- The next tRNA pairs with the next mRNA codon which carries another amino acid.
- Two amino acids form peptide bonds.
- Ribosome moves to third codon and so on.