DNA DS break Repair Flashcards
how many double strand breaks are required to kill a cell?
one
What are the 2 categories of double strand breaks?
endogenous and exogenous
what are the 5 ways that double strand breaks occur?
- immune system rearrangements
- single strand breaks during DNA replication
- meiosis
- ionizing radiation (cosmic rays and soils)
- medical imaging and treatments
what are the 2 ways double strand breaks are repaired?
- non -homologous end joining
2. homologous recombination
what determines whether repair occurs by NHEJ or HR?
where the cell is in the cell cycle and what type of break is present, if sister chromatid is present (HR)
what 2 sections are joined together in the light chain of immune cells?
V and J
what 2 sections are joined together in the heavy chain of immune cells?
D and J
what is the main contributions to the diversity of antibody diversity?
Non-homologous end joining
what initiates NHEJ?
recognition of break by Ku
what does Ku recruit?
DNA-protein kinase (DNA-PKcs)
what is the purpose of artemis?
make a cut in the DNA so that hopefully it can be ligated back together
What DS break repair system is used in meiosis?
homologous recombination
what causes a stalled replication fork?
single strand break
what does wortmannin inhibit?
PI-3 kinases
what 2 proteins are recruited during double strand breaks?
Rad51 and Brca1
