DNA and RNA

Question 1

the first to isolate DNA in 1869

Answer 1

Friedrich Miescher

Question 2

credit grabbers who introduced kuno the DNA

Answer 2

Francis Crick & James Watson

Question 3

purines (2)

Answer 3

adenine and guanine

Question 4

pyrimidines (2)

Answer 4

cytosine and thymine

Question 5

building blocks of dna

Answer 5

nucleotides

Question 6

basic structure of nucleotides

Answer 6

sugar phosphate backbone
nitrogenous base
base pair

Question 7

transfers the genetic code from the nucleus to the

ribosomes during protein synthesis

Answer 7

RNA

Question 8

important roles of RNA (3)

Answer 8

protein synthesis
cellular signals
catalyst

Question 9

sugar in DNA

Answer 9

deoxyribose

Question 10

sugar in RNA

Answer 10

ribose

Question 11

Responsible for carrying information about a protein

sequence to the ribosomes

Answer 11

mRNA

Question 12

Transfers amino acids to growing polypeptide chains at

the ribosomal site of protein synthesis

Answer 12

tRNA

Question 13

Comprise majority of the RNA found among typical

eukaryotic cells

Answer 13

rRNA

Question 14

collection of DNA with higher yield

Answer 14

blood

Question 15

preferred tubes for blood and bone marrow (2)

Answer 15

lavender - EDTA

yellow - acid citrate dextrose

Question 16

preferred tube for isolation of plasma

Answer 16

white - K2EDTA with gel barrier

Question 17

isolation of mononuclear cells:

enhanced WBC recovery:

Answer 17

blue/black - sodium citrate, gel, density gradient fluid

yellow - acid citrate dextrose

Question 18

this tube is not recommended to use since it inhibits polymerase

Answer 18

green - heparin

Question 19

ways to expose nucleic acid of bacteria and fungi: (4)

Answer 19

• enzymes
• grinding w/ glass beads
• chem tx
• commercial rgt kits