BIOSAFETY

Question 1

Any microbial entity, cellular or non-cellular, naturally
occurring or engineered, capable of replication or of
transferring genetic material that may be able to
provoke infection, allergy, toxicity, or other adverse
effects in humans, animals, or plants

Answer 1

biological agent

Question 2

Any material comprised of, containing, or that may
contain biological agents and/or their harmful products,
such as toxins and allergens

Answer 2

biological material

Question 3

bacteria, fungi, viruses, viroids,
endo/ectoparasites are examples of:

a. biological agents
b. biological material

Answer 3

biological agents

Question 4

PPEs and laboratory materials are examples of:

a. biological agents
b. biological material

Answer 4

biological material

Question 5

Potential source of harm caused by biological

materials

Answer 5

biohazard

Question 6

Containment principles, technologies, and practices
implemented to prevent unintentional/accidental
exposure to pathogens and toxins, or their
unintentional/accidental release

Answer 6

biosafety

Question 7

Institutional and personal security measures designed to prevent the loss, theft, misuse, diversion, or intentional release of pathogens and toxins

Answer 7

biosecurity

Question 8

Protecting people from bad bugs.

Answer 8

biosafety

Question 9

Protecting bad bugs from bad people.

Answer 9

biosecurity

Question 10

recapping of needles

a. biosafety issue
b. biosecurity issue

Answer 10

biosafety issue

Question 11

lab tech is a member of terrorist group

a. biosafety issue
b. biosecurity issue

Answer 11

biosecurity issue

Question 12

lab tech not provided with PPEs

a. biosafety issue
b. biosecurity issue

Answer 12

biosafety issue

Question 13

lab wastes are not segregated

a. biosafety issue
b. biosecurity issue

Answer 13

biosafety issue

Question 14

lab tech with huge debt

a. biosafety issue
b. biosecurity issue

Answer 14

both

Question 15

laboratory biosafety 3Ps:

Answer 15

PROTECT USERS OF THE LABORATORY
PROTECT THOSE OUTSIDE THE LABORATORY
PROTECT THE ENVIRONMENT

Question 16

most important concept in principles of biosafety

a. practices and procedures
b. standard practices
c. special practices and considerations

Answer 16

standard practices

Question 17

primary containment barrier

a. safety equipment
b. facility design and construction

Answer 17

safety equipment

Question 18

secondary barrier/engineering controls

a. safety equipment
b. facility design and construction

Answer 18

facility design and construction

Question 19

identify the biosafety levels:

lab type: teaching/research
safety eq: none

Answer 19

basic - biosafety level 1

Question 20

identify the biosafety levels:

lab type: primary health/diagnostic service/research
safety eq: BSC

Answer 20

basic - biosafety level 2

Question 21

identify the biosafety levels:

lab type: special diagnostic service/research
safety eq: BSC

Answer 21

containment - biosafety level 3

Question 22

identify the biosafety levels:

lab type: dangerous pathogens
safety eq: BSC III, airlock, shower exit

Answer 22

maximum containment - biosafety level 4

Question 23

Any material that contains or has been contaminated by a biohazardous agent

a. biological wastes
b. cultures/infectious agents
c. pathological wastes
d. isolation wastes
e. pharmaceutical wastes

Answer 23

biological wastes

Question 24

Before discarding, they must be decontaminated

a. biological wastes
b. cultures/infectious agents
c. pathological wastes
d. isolation wastes
e. pharmaceutical wastes

Answer 24

cultures/infectious agents

Question 25

Human surgery specimen or tissues, including those
fixed in fixatives

a. biological wastes
b. cultures/infectious agents
c. pathological wastes
d. isolation wastes
e. pharmaceutical wastes

Answer 25

pathological wastes

Question 26

generated by hospitalized patients
who are isolated to protect others from communicable
diseases

a. biological wastes
b. cultures/infectious agents
c. pathological wastes
d. isolation wastes
e. pharmaceutical wastes

Answer 26

isolation wastes

Question 27

expired, unused,
split/contaminated pharm products, drugs, vaccines, and
sera

a. biological wastes
b. cultures/infectious agents
c. pathological wastes
d. isolation wastes
e. pharmaceutical wastes

Answer 27

pharmaceutical wastes

Question 28

mixed waste hierarchy (highest to lowest)

Answer 28

radioactive > chemical > biological

Question 29

T or F. Whenever there is a radioactive waste present, treat the
waste as radioactive

Answer 29

true

Question 30

waste management process (5)

Answer 30

1. segregation
2. packaging
3. handling and storage
4. transportation
5. treatment and disposal

Question 31

most important step, key to any effective waste management

Answer 31

segregation

Question 32

color coding for wastes:

non-infectious, dry waste

Answer 32

black

Question 33

color coding for wastes:

non-infectious, wet waste

Answer 33

green

Question 34

color coding for wastes:

infectious waste

Answer 34

yellow

Question 35

color coding for wastes:

chemical and pharmaceutical waste

Answer 35

yellow with black band

Question 36

color coding for wastes:

radioactive waste

Answer 36

orange

Question 37

special metal used to contain radioactive waste

Answer 37

lead

Question 38

treatment of biological waste:

for culture plates

Answer 38

autoclaving

Question 39

treatment of biological waste:

when there is moisture in the waste

Answer 39

microwave irradiation

Question 40

treatment of biological waste:

uses enzyme mixture

Answer 40

biological process

Question 41

treatment of biological waste:

breaking the DNA molecules

Answer 41

radiation process

Question 42

treatment of biological waste:

mixing waste with immobilizing material

Answer 42

encapsulation

Question 43

what wastes are being subjected for encapsulation?

Answer 43

sharps and pharmaceuticals