BIOSAFETY Flashcards
Any microbial entity, cellular or non-cellular, naturally
occurring or engineered, capable of replication or of
transferring genetic material that may be able to
provoke infection, allergy, toxicity, or other adverse
effects in humans, animals, or plants
biological agent
Any material comprised of, containing, or that may
contain biological agents and/or their harmful products,
such as toxins and allergens
biological material
bacteria, fungi, viruses, viroids,
endo/ectoparasites are examples of:
a. biological agents
b. biological material
biological agents
PPEs and laboratory materials are examples of:
a. biological agents
b. biological material
biological material
Potential source of harm caused by biological
materials
biohazard
Containment principles, technologies, and practices
implemented to prevent unintentional/accidental
exposure to pathogens and toxins, or their
unintentional/accidental release
biosafety
Institutional and personal security measures designed to prevent the loss, theft, misuse, diversion, or intentional release of pathogens and toxins
biosecurity
Protecting people from bad bugs.
biosafety
Protecting bad bugs from bad people.
biosecurity
recapping of needles
a. biosafety issue
b. biosecurity issue
biosafety issue
lab tech is a member of terrorist group
a. biosafety issue
b. biosecurity issue
biosecurity issue
lab tech not provided with PPEs
a. biosafety issue
b. biosecurity issue
biosafety issue
lab wastes are not segregated
a. biosafety issue
b. biosecurity issue
biosafety issue
lab tech with huge debt
a. biosafety issue
b. biosecurity issue
both
laboratory biosafety 3Ps:
PROTECT USERS OF THE LABORATORY
PROTECT THOSE OUTSIDE THE LABORATORY
PROTECT THE ENVIRONMENT
most important concept in principles of biosafety
a. practices and procedures
b. standard practices
c. special practices and considerations
standard practices
primary containment barrier
a. safety equipment
b. facility design and construction
safety equipment
secondary barrier/engineering controls
a. safety equipment
b. facility design and construction
facility design and construction
identify the biosafety levels:
lab type: teaching/research
safety eq: none
basic - biosafety level 1
identify the biosafety levels:
lab type: primary health/diagnostic service/research
safety eq: BSC
basic - biosafety level 2
identify the biosafety levels:
lab type: special diagnostic service/research
safety eq: BSC
containment - biosafety level 3
identify the biosafety levels:
lab type: dangerous pathogens
safety eq: BSC III, airlock, shower exit
maximum containment - biosafety level 4
Any material that contains or has been contaminated by a biohazardous agent
a. biological wastes
b. cultures/infectious agents
c. pathological wastes
d. isolation wastes
e. pharmaceutical wastes
biological wastes
Before discarding, they must be decontaminated
a. biological wastes
b. cultures/infectious agents
c. pathological wastes
d. isolation wastes
e. pharmaceutical wastes
cultures/infectious agents
Human surgery specimen or tissues, including those
fixed in fixatives
a. biological wastes
b. cultures/infectious agents
c. pathological wastes
d. isolation wastes
e. pharmaceutical wastes
pathological wastes
generated by hospitalized patients
who are isolated to protect others from communicable
diseases
a. biological wastes
b. cultures/infectious agents
c. pathological wastes
d. isolation wastes
e. pharmaceutical wastes
isolation wastes
expired, unused,
split/contaminated pharm products, drugs, vaccines, and
sera
a. biological wastes
b. cultures/infectious agents
c. pathological wastes
d. isolation wastes
e. pharmaceutical wastes
pharmaceutical wastes
mixed waste hierarchy (highest to lowest)
radioactive > chemical > biological
T or F. Whenever there is a radioactive waste present, treat the
waste as radioactive
true
waste management process (5)
- segregation
- packaging
- handling and storage
- transportation
- treatment and disposal
most important step, key to any effective waste management
segregation
color coding for wastes:
non-infectious, dry waste
black
color coding for wastes:
non-infectious, wet waste
green
color coding for wastes:
infectious waste
yellow
color coding for wastes:
chemical and pharmaceutical waste
yellow with black band
color coding for wastes:
radioactive waste
orange
special metal used to contain radioactive waste
lead
treatment of biological waste:
for culture plates
autoclaving
treatment of biological waste:
when there is moisture in the waste
microwave irradiation
treatment of biological waste:
uses enzyme mixture
biological process
treatment of biological waste:
breaking the DNA molecules
radiation process
treatment of biological waste:
mixing waste with immobilizing material
encapsulation
what wastes are being subjected for encapsulation?
sharps and pharmaceuticals
