Dna Flashcards

1
Q

what is dna made of

A

phosphate group , pentose sugar (deoxyribose) and a nitrogen-containing base

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2
Q

how are the nucleotides joined together?

A

my phosphodiester bonds between the sugar and another nucleotides phosphate group by condensation

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3
Q

what is DNA polymerase

A

an enzyme which creates the phosphodiester bond

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4
Q

describe the structure of DNA

A

a double helix structure which has two polynucleotide chains joined by hydrogen bonds between the base pairs

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5
Q

describe the bonding between the bases

A

adenine forms 2 hydrogen bonds with thymine and guanine forms 3 hydrogen bonds between cytosine

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6
Q

what does anti parallel mean ?

A

the strands run in opposite directions of each-other
one strand starts with C5 and ends with C3
another starts with C3 and ends with C5
Reads it in the 5 to 3 direction

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7
Q

what is the function DNA replication

A

to create more copies so that when cells divide they have exact copies of DNA

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8
Q

what is the function of protein synthesis?

A

contains genes which code for the order of amino acids in a protein

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9
Q

describe the structure of DNA and its advantages

A

long - stores a lot of information
helical - compact
double stranded - each stand acts as a template for DNA replication
sugar phosphate backbone - provides strength and stability and protects the bases within the helix
weak hydrogen bonds- easily broken and unzipped for DNA replication or protein synthesis
lots of hydrogen bonds - makes the molecule stable and strong

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10
Q

describe the nucleotide of RNA

A

single molecule , relatively short
made up of phosphate group , ribose pentose sugar and nitrogen containing base

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11
Q

describe mRNA and its role in

A

single stranded straight chain molecule made of bases which are read in co-dons
role is to carry the genetic code out of the nucleus to the ribosome for the formation of 1 polypeptide chain

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12
Q

describe tRNA and its role

A

single strand of RNA folded into many places forming complementary base pairs
cover leaf shape
has an amino acid binding site and a triplet of bases called an anticodon complementary to mRNA codon
role is to carry specific amino acids to the mRNA at ribosomes for formation of polypeptide chain

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13
Q

describe RRNA and its role

A

rRNA is found in the cytoplasm
role- along with a protein molecule it forms ribosomes

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14
Q

DNA VS RNA

A

DNA
two strands
very long
thymine
stores genetic information
RNA
one strand
relatively short
uracil
transfers genetic information in the formation of proteins and helps form ribosomes

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15
Q

what’s the stage during the cell cycle where DNA replication happens

A

interphase

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16
Q

what does semi conservative mean?

A

means that each strand acts as a template and that each new molecule of dna contains one original strand and one new

17
Q

DNA helicase

A

unwinds DNA and breaks hydrogen bonds between bases allowing nucleotides to attach to each strand and for each stand to act as a template

18
Q

DNA polymerase

A

joines adjacent nucleotides together forming phosphodiester bonds in the sugar-phosphate backbone

19
Q

stages of DNA replication

A
  1. dna helicase separates DNA by breaking hydrogen bonds
  2. each strand acts as template
  3. free nucleotides attach to the exposed bases by complementary base pairing
  4. DNA polymerase joins nucleotides forming a phosphodiester bond
20
Q

how is DNA replicated direction wise

A

5th carbon to 3rd carbon
polymerase enzymes have an active site which is complimentary and specific to the 3rd carbon end of the chain
this means that new nucleotides are added in the 5th carbon to 3rd carbon direction