Dna 16-18 Flashcards

1
Q

Dna synthesize from

A

5’ to 3’

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2
Q

What is the term topoisomerase

A

It is when the DNa has many forms

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3
Q

Helicase function

A

Unzip dna for initial insertion

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4
Q

Exonuclease function

A

To clip out the primers

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5
Q

Primers are made from what

A

Rna

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6
Q

What connects the gaps between dna fragments

A

Ligase

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7
Q

Whats a dna cap

A

Telomere (shows the end of the strand)

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8
Q

Po3 function

A

Enlongation

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9
Q

Po1 function

A

Clean primers, and fill in the gaps

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10
Q

Common function between po1 and po3

A

Can both proofread

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11
Q

Types of mutation

A

Substitution, deletion and insertion

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12
Q

What checks for mutation

A

MuTL (checks the methylation of the dna)

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13
Q

What removes the mutation

A

MUTH (removes and ligase would connect the back)

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14
Q

Muts function

A

Proofread again

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15
Q

Uc effect on dna

A

They can cause the cross link of the thymine. Remove by phosphorylation

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16
Q

Whats translesion

A

Forcing the completion eventhough theres an error cause it cannot be fixed

17
Q

Po4 and 5 function

A

Po4 would overlook a damage whereas po5 would not

18
Q

Whats a double base strand repair

A

If the strand is homo= make a new strand
If the strand is hetero= ligase fix

19
Q

function of tata

A

dictate the replication direction

20
Q

steps to regulation of gene

A

sequence -> promoter -> tata

21
Q

positive control eg

A

lac opéron

22
Q

negative control

A

trp operon

23
Q

what are opérons

A

a cluster of gene that is controlled via on or off switch. responsible for many related expressions

24
Q

name bacteria opérons

A

lac z, lac y, lac a

25
Q

lac opérons should normally be

A

off

26
Q

function of operator

A

the in or off switch

27
Q

how does lac op. works

A

once lactose is present in the system, binds to the repressor to remove them. the synthesis runs and proteins are made to digest the lactose

28
Q

can lac be positive

A

yes. according tô the amount of lac in the lac1

29
Q

how many trp proteins are there

A

5

30
Q

trp is normally on or off

A

on

31
Q

transcription factor pathway

A

protein binds promoter then to enchaine then the synthesis can take place

32
Q

what makes trp turns off

A

triptrophan

33
Q

what happened when there not triptrophan

A

operator works (always on) and triptrophan is made