Dna Flashcards
What do nucleic acids do
Store information that controls cellular activity
What di nucleic acids control
Protein synthesis and chemical processes
Where is dna found
Nucleus
Two types of nucleic acid
Deoxyribo nucleic acid
Ribonucleic acid
What does dna form
An important part of the chromosomes that form the chromatin network
What is a gene
Secrions of dna molecules that contain hereditary info
Structure of dna
Doible helix
1 sugar
1 phoshate
1 nitro base
What are the nitro bases
Adenine with thymine
Cytosin with guanine
Which of the nitro bases are pyrimidines
Thymine and cytosine
Which of the nitro bases are purines
Adenine and guanine
What is dnas role
Hereditary information in the form of genes
Coding for protein
to replicate
Coding dna is called what
Exons
Non coding dnas other name and fuctions
Introns and dunctional rna molecules
Why is dna replication important
Make new dna molecule to ensure the genetic code is passed on to each daughter cell
When does dna relication take place
Interphase
How does dna replication take place
1 double helix unwinds,weak hydro break
2 exposed strand =template
3 free nucleotides bond to base pair
=two identical double helix wrap around histone
What is an str
Short tandem repeat
In dna profiling where do the differences occur
The non coding part
What is dna profilling
The extracting if highly variable regions =repeating sequences of base pairs
How mang diffrent site of dna profilling are investigated
13-20
What is a dna profile
An individuals unique dna fragments,seperated by electrophoresis
How does dna profilling occur
Cells treated to extract dna
Restriction enzymes cut begin and end parts
Dna gets amplified
Electrophoresis
Whatbis gel electrophoresis
A method to sepreate latge molecules mainly due to size and electrical charge
Hiw is dna profilling used
Forensics
Didorders
Paternity
What is the strictute of rna
Single stranded
Made of nucleotides
1 sugar 1 base 1 phosphate
What is the function of rna
Carties instructions from dna in nucleus to ribosomes for protein synthesis
What are the similarities of dna and rna
Made of polymers
Same structure base phospate and sugar
Both responsible for protein synth
What are the types of rna
Messenger rna
Transfer rna in cyto
Ribosomal RNA
What happens in the nucleus
Trnscription
Dna trabscribed into new mrna molecule
Gene unwinds nucleotides pair up/join to make rna
The order of codons equals what
The order of amino acids
The triplet code is the basis for what
Genetic code
What happens at the ribosomes
Mrna binds to ribosome at start codon and trna binds one amino to one mrna to deposit aminos correct
What is the anti codon
The three notrogenous bases at the end of a trna molecule
What is translation
One codon starts the process
Anti codons base link to compl bases
Mrna translated into amino sequence
Translation definition
The process by which a specific protein is formed from a chain of amino acids dude too the sequence of codons
What is the purpose of rrna
Firm ribosomes
Read the genetic code
What are the basic components of a codon and what does it code for
3 nitro bases
And protein
What are the basic components of a gene and whatvdoes it code for
Nirto bases
Anti codon
Why do we squish the strawberries
Break the cell walls
Why do we use a soap and salt mix
Detergent causes the cell mems to break down
And salt enable the nucleic asiv to precipitate
Why do we use heat treatment
Break down phospholipids to denature enzymes
Why do we use a cold bath
Slow down the rate at which the dna breaks down
Filtering is to allow what
The small dna moleciles to pass through
Chilled ethanol
Dissolves the soluble components to form a solution
Precipitaion is for
To allow the insoluble molecules to clump toegether
Gmo plants techniques
1 gene gun
2 recombinant dna technique
What dies a gene gun do
Shoots a dna covered bullet in to the desirable part of the plant cells
Recombinant dna how does it happen
T1 recombinant removed and restriction enzyme cuts plasmid
Ligase splices the gene =transgene
=intergration=grown by tissue culture
Advantages of gmo tech
Saving in pest and diseas control
Higher yields
Disadnvatages of gmo tech
Threat to biodiversity
Disrupt notmal gene function
Genetic abnormalities
What are the 3 types of cloning
1 gene cloning using a plasmid
2 reproductive cloning
3 therepeutic cloning
What are the nuclear transfer techniques
1 embryonic nuclear transfer
2 somatic cell transfer
What is embryonic nuclear transfer
Nuckear transfer directly from embryos
How does somatic cell transfer occur
1donir cell cultures in low nutrient medium
2 nucleus of egg sucked by micropipet
3 electric pulse connects
4 electirc pulse fuses then another =cell divions
5 embryo in mom then gestation and birth
How can cloning be used
For envirtro
New organs /cells
Tissue replacement
What is external fertilisation
Huge numbers
Mainly aquatic
Not certain
Internal fertilisation what is it
Terrestrial
Birds and reptile =cloaca
Most mammal male penis
Sperm to femal =copulation
What is ovipary
Eggs develop outside
Egg yolk =food
What is ovivipary
Eggs inside till hatch
What is vivipary
Internal
No egg shell
Placenta
What is a Cloaca
Single opening in lower abdomen
Precocial
More energy before birth
Can survive on own
Less parental care
Altricial
Not well developed
Needs parebtal care