Disinfection/sterilisation

Question 1

What is disinfection

Answer 1

Decontamination process that aims to kill or inactivate harmful microorganisms, but LEAVES some microbes INTACT

Question 2

What is sterilisation

Answer 2

Decontamination process that aims to kill ALL microorganisms

Question 3

4 properties of a disinfectant to use in your clinic

Answer 3

Broad spectrum - wide antimicrobial disease
Fast acting - kill rapidly
Non toxic - not harm user or patients
Leave a residual effect on treated surfaces

Question 4

How would you conveniently test effectiveness of your autoclave weekly

Answer 4

Spore test
(need explain how done) - I don’t know
From google:
Select 2 ampoules from the same lot number.
One used in the sterilizer, other will be the control so remains outside the sterilizer to check that the spores in the lot were not defective

Place the test ampoule in the autoclave and run the autoclave
Remove the ampoule from the autoclave

Crush the test and control ampoules to release the enzyme that causes an enzymatic reaction and therefore fluorescence

Place both ampoules in the incubator for the indicated time
Read the results for the test and control.=
‘pass’ result, if the spores from the test vial are killed and the spores from the control vial are alive.

Question 5

Name 2 organisms a clinical disinfectant should be tested against to ensure efficacy

Answer 5

Gram positive bacterium (Staphylococcus aureus)
Gram negative bacterium ( SalmonellaTyphi )