Disinfection and Sterilization Flashcards
Define: disinfect
Reduce pathogenic organism count to safe level
Define: sterilize
Inactivate all microbes including spores
What is autoclaving and what does it do?
Pressurized steam >120 C
Sporicidal
May not reliably inactivate prions
What do alcohols do in disinfection/sterilization?
Denature proteins and disrupt cell membranes. Not sporicidal.
What does chlorhexidine do in disinfection/sterilization?
Denature proteins and disrupt cell membranes. Not sporicidal.
What does chlorine do in disinfection/sterilization?
Oxidizes and denatures proteins
Sporicidal
What does hydrogen peroxide do in disinfection/sterilization?
Free radical oxidation
Sporicidal
What does iodine do in disinfection/sterilization?
Halogenation of DNA, RNA, and proteins
May be sporicidal
What do quaternary amines do in disinfection/sterilization?
Impair permeability of cell membranes
Not sporicidal