Disinfection and Sterilization Flashcards

1
Q

Define: disinfect

A

Reduce pathogenic organism count to safe level

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2
Q

Define: sterilize

A

Inactivate all microbes including spores

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3
Q

What is autoclaving and what does it do?

A

Pressurized steam >120 C
Sporicidal
May not reliably inactivate prions

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4
Q

What do alcohols do in disinfection/sterilization?

A

Denature proteins and disrupt cell membranes. Not sporicidal.

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5
Q

What does chlorhexidine do in disinfection/sterilization?

A

Denature proteins and disrupt cell membranes. Not sporicidal.

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6
Q

What does chlorine do in disinfection/sterilization?

A

Oxidizes and denatures proteins

Sporicidal

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7
Q

What does hydrogen peroxide do in disinfection/sterilization?

A

Free radical oxidation

Sporicidal

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8
Q

What does iodine do in disinfection/sterilization?

A

Halogenation of DNA, RNA, and proteins

May be sporicidal

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9
Q

What do quaternary amines do in disinfection/sterilization?

A

Impair permeability of cell membranes

Not sporicidal

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