Digestion Lab

Question 1

The ____ produces pepsin which, in the presence of hydrochloric acid, digests protein.

Answer 1

stomach

Question 2

____ is a hydrolytic enzyme that breaks starch down to maltose.

Answer 2

Salivary amylase

Question 3

A ______ is made in order to compare a known standard to an experimental standard.

Answer 3

controls

Question 4

An enzyme has a pocket or ______, which the substrate or substrates must fit into temporarily in order for catalysis to occur.

Answer 4

Active site

Question 5

During digestion, the chief cells of the stomach secrete ____ which is responsible for the digestion of protein.

Answer 5

Pepsin

Question 6

Positive or Negative controls are used to determine whether there are any contaminating substances in the reagents used in an experiment.

Answer 6

Negative

Question 7

Digestive system

Answer 7

consists of the digestive tract and accessory glands that secrete enzymes and fluids needed for digestion.

Question 8

Salivary glands

Answer 8

produce salivary amylase for the digestion of starch.

Question 9

stomach glands

Answer 9

produce pepsin (in the presence of HCl) for the digestion of proteins

Question 10

Pancreas

Answer 10

produces pancreatic lipase for the digestion of fats, and pancreatic amylase for the digestion of starch (enzymes are ducted to the small intestine).

Question 11

Liver

Answer 11

Produces bile for the emulsification of fats (ducted to the small intestine).

Question 12

Small intestine

Answer 12

produces enzymes for digestion of starch and proteins.

Question 13

Enzymes

Answer 13

are large protein molecules produced by body cells.

Question 14

Describe the significance of using a 37 Celsius incubation temp. to test salivary amylase activity.

Answer 14

It is significant because it is the same as body temp.

Question 15

Describe the usual substrate for peptidase.

Answer 15

It’s peptides and proteins.

Question 16

Explain why pancreatic lipase would be active in both the mouth and the intestine.

Answer 16

The pancreatic lipase enzyme has the highest enzymatic activity in environment around pH 7. The mouth had pH around 7 and small intestine around 8, so the enzyme would work in both places.