Digestion Flashcards

1
Q

Most molecules are too…

A

large to pass through absorbing surface of gut wall

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2
Q

Function + source of CARBOHYDRATES

A

-source of energy
-glucose is main respiratory substrate
STARCH + SUGAR

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3
Q

Function + source of PROTEINS

A
  • growth and repair

- meat eggs cheese beans

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4
Q

Lipids - function and source

A

Energy - make up most of the cell membrane

butter, margarine, meat

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5
Q

Glucose

A
  • form of starch - starch is a polymer of glucose

small enough to be absorbed through walls of digestive system

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6
Q

Cellulose

A

cannot be broken down by digestive system

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7
Q

Proteins

A

proteins too big to pass through walls of digestive system

  • broken down to amino acids
  • protein synthesis, happens in ribosomes of cells
  • excess amino acids broken down in liver
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8
Q

Lipids

A

ester of fatty acids and glycerol

-used for cell membranes

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9
Q

What does qualitative mean

A

results not expressed as numbers of graphs. represented by colour or presence

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10
Q

Test for sugars ? and method ?

A

Benedictus test
-add + benedict’s solution to food
-heat in a water bath
- presence of sugar - orange or brick red
depending on how much glucose is present it will go through stages - green, yellow, orange, red

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11
Q

HAZARDS

A

wear safety goggles
benedict’s solution is an irritant
avoid contact with skin and eyes

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12
Q

test for starch ? + method

A

+ add iodine solution to food

  • food containing starch will turn blue-black
  • can use microscope to stain starch grains in plants
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13
Q

HAZARDS

A
  • safety goggles
  • Iodine solution is an irritant
  • avoid contact with skin and eyes
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14
Q

Test for proteins ? method ?

A

BIURET’S TEST

  • use biuret’s reagent A + B
  • add 1cm cubed of biurets solution A to food SOLUTION
  • mix liquids
  • add 1cm cubed of buiret’s solution B and shake
  • PURPLE colour indicates protein is presetn
  • A purple ring between layers indicates protein is present
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15
Q

HAZARDS

A

Safety goggles
Buiret’s solution A is CORROSIVE
Buiret’s solution B is an irritant
avoid contact with skin and eyes

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16
Q

TEST FOR FATS + METHOD

A

SUDAN III test
- equal amounts of food + water added to test tube
- a few drops of sudan III added and shake tube
RESULTS - Red stained layer forms above surface of water

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17
Q

The alternative test for lipids/fats

A

Emulsion test

  • ethanol added to test tube with crushed food
  • liquid poured into another test tube containing water, leaving any food residue behind
  • RESULTS - CLOUDY LIQUID INDICATES PRECENSE OF LIPIDS
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18
Q

HAZARD..

A

Ethanol is flammable

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19
Q

Human digestive system purpose ?

A

breaks down complex food substances

provides large surface area for maximum absorption

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20
Q

what is the digestive system ?

A

Organ system which breaks down food so it can be absorbed by the bloodstream

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21
Q

function of mouth

A

begins digestion of carbohydrates
saliva contains AMYLASE which begins to break down starch into sugars
saliva also contains lipase - breaks down fats

22
Q

function of stomach

A

begins digestion of protein - small molecules such as alcohol absorbed
contains AMYLASE, PROTEASE ( peptides + amino acids ), LIPASE ( fatty acids and glycerol )

23
Q

function of small intestine ?

A

completes digestion of carbohydrates and proteins into SINGLE SUGARS and AMINO ACIDS and FATTY ACIDS and GLYCEROL
-contains

24
Q

Large intestine

A

absorption of water - eggestion of undigested food

25
how is villi in gut wall adapted for digestion
surface of wall is folded and has villi - large surface area - epithelial cells that cover each villus have projections called microvilli - network of blood capilleries - easy absorbtion, maintains concentraion gradient - digested food passes through epithelial cells of gut wall and carried by blood to liver
26
what are enzymes
biological catalysts that are proteins with a complex shape. Has a region called the active site which is specific to the substrate that fits to it
27
substrate ?
molecule taking part in reaction
28
enzyme reaction
- substrate collides with active site and attaches - active site has complementary shape to substrate - enzyme catalyses breakdown of substrate - enzyme molecule is unchanged and can be used again
29
factors affecting enzyme action
low temp - molecular movement decreases high temp - at optimum temp 37 degrees hight temp - also disrupts shape of active site which reduces its activiity as it no longer fits with substrate and so the enzyme is denatured.
30
enzyme action
enzymes made of folding chains of amino acids which is held together by weak forces between amino acid molecules in the chain. If temp is too high, rate of reaction will be affected because the shape has changed.
31
effect of ph
amino acids carry a charge in an enzyme molecule - negative and positive attract changing ph will affect the shape of the chains of folding amino acids can become denatured
32
cellular enzymes work best at ph....
7
33
how does the stomach create the optimum ph for enzymes
secretion of hydrochloric acid
34
optimum ph in small intestine is created by....
by secreting sodium hydrogen carbonate
35
optimum ph for salivary amylase
6.8
36
optimum ph for stomach protease ( pepsin )
1.5 - 2
37
optimum ph for pancreatic protease
7.5 - 8
38
carbohydrase
breaks carbohydartes into simple sugars
39
amylase
breaks starch into glucose
40
protease
breaks proteins down into amino acids
41
lipase
breaks lipids down into fatty acids and glycerol
42
MOUTH
starch - maltose ( amylase catalyst )
43
small intestine
amylase produced in pancreas | starch - maltose ( pancreatic amylase )
44
STOMACH
pepsin produced in gastric glands | proteins - amino acids (pepsin catalysts)
45
small intestine
protease ( trypsin ) produced in pancreas | protein - amino acids
46
small intestine ( lipases )
lipids - fatty acids + glycerol ( lipase produced in pancreas )
47
required practical - investigate effect of ph on enzyme amylase actvity
buffer solutions remain at the same ph even when acids and alkalis are added 1. place one drop of iodine solution into each well of spotting tile 2. 3 test tubes 3. 1st test tube - add 3cm cubed of starch solution 4. 2nd test tube - 2cm cubed of amylase solution 5 3rd test tube - 2cm cubed of ph 5 buffer solution - controls ph 6. place all 3 test tubes in water bath at 30 degrees for 10 mins 7. combine all three test tubes into one solution - mix with a stirring rod 8. return to the water bath and start timer 9. after 30 secs, transfer one drop of solution to a spotting tile iodine should turn blue black if starch is present 10. take a sample every thirty seconds until the iodine remains orange - starch is no longer present in solution 11. record time to see the rate of amylase at ph 5 12. repeat experiment with different ph buffers 6, 7 , 8
48
problems
taking samples every 30 seconds - only an approximate time for reaction to complete, just an indication of rate. it is the INVERSE of the rate so we can do ONE / TIME TAKEN to work out a more accurate answer take it every 10 seconds instead colour change tends to be gradual ask several people to look at spotting tile to decide if the reaction has completed
49
safety
wear goggles amylase may cause allergic reactions iodine solution is an irritant
50
effect of temp
use a water bath to maintain temps with different test tubes breakdown of lipids by lipase - monitor ph change - as reaction proceeds, release of fatty acids would mean ph decreases
51
liver and digestion
liver produces bile - emulsifies lipids into tiny droplets which have a bigger surface area for the lipds to work on. contains - sodium hydrocarbonate - alkali - neutralises stomach acid - optimum ph bile produced in liver - stored in gal bladder