Diagnostics

Question 1

fungi sampling tip

Answer 1

sample from the periphery of the lesion (center may have bacterial contamination)

Question 2

Diff quick

Answer 2

Mycoplasma haemofelis

Question 3

DCF

Answer 3

campylobacter

Question 4

Polychrome methylene blue

Answer 4

bacillus anthracis

Question 5

modified ziehl-nielsen

Answer 5

brucella abortus

Question 6

Ziehl-nielsen

Answer 6

mycobacterium

Question 7

direct microscopic exam provides info on:

Answer 7

number of bacteria/fungi, morphology, host cell response

Question 8

direct microscopic exam limits:

Answer 8

requires expertise, sensitivity of direct staining is low, usually does not allow species ID

Question 9

Culture media

Answer 9

contains essential nutrients for growth of general bacteria (non specific nutritional requirements)

Question 10

selective media

Answer 10

for growth of only selected bacteria

Question 11

Phenylethyl alcohol sugar (PEA) media selective for

Answer 11

Gram + bacteria

Question 12

MacConkey Agar selective for

Answer 12

gram - bacteria

Question 13

Sabourad’s dextrose agar selective for

Answer 13

fungal cultures

Question 14

Differential media

Answer 14

distinguish one bacteria growing on the same plate from another

Question 15

Blood agar differentiates

Answer 15

which bacteria produce hemolysin

Question 16

MacConkey agar differentiates

Answer 16

bacteria based on nutrient utilization. lactose fermentation turns purple, non-lactose fermentation is yellow

Question 17

Serology advantages

Answer 17

widely used in screening populations, rapid tests available, some tests useful for carriers

Question 18

serology limitations

Answer 18

false negatives, false positives