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Bioanalytical Pathology > Diagnostic Immunology > Flashcards

Diagnostic Immunology Flashcards

1
Q

What are the most common types of lymphoma/leukemia in dogs and cats?

A

Acute leukemia
Peripheral T cell lymphoma
Diffuse large B cell lymphoma
B cell chronic lymphocytic leukemia
Large granular lymphocytic leukemia
T zone lymphoma/leukemia

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2
Q

What antibodies are identified by flow cytometry for different canine lymphoproliferative disorders?

A

T cells: CD3, CD5
T cell subsets: CD4 or CD8
B cell: CD21
Immature (acute leuk): CD34
All leukocytes: CD45

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3
Q

What antibodies are identified by flow cytometry for different feline lymphoproliferative disorders?

A

CD4, CD8, CD5, CD21

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4
Q

What lymphoproliferative disorders have a poor prognosis?

A

Acute leukemia
Peripheral T cell lymphoma

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5
Q

What lymphoproliferative disorders have a good prognosis?

A

Diffuse large B cell lymphoma
B cell chronic lymphocytic leukemia
Large granular lymphocytic leukemia
T zone lymphoma/leukemia

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6
Q

List the main differentials for mature lymphocytosis in the peripheral blood of a dog.

A

Chronic lymphocytic leukemia
Chronic Ehrlichia canis infection
Addison’s disease
Thymoma

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7
Q

Describe the principles of flow cytometry, including light scatter and use of monoclonal antibodies for immunophenotyping.

A

Distinguishes a neoplastic from a reactive/non-neoplastic population of lymphocytes because neoplastic lymphocytes are homogenous

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8
Q

What should be sent with lymphocytosis?

A

Blood

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9
Q

What should be sent with lymphadenopathy?

A

LN aspirate

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10
Q

Pros of flow cytometry

A
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11
Q

Cons of flow cytometry

A
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12
Q

Pros of PARR

A
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13
Q

Cons of PARR

A
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14
Q

Compare flow cytometry, PARR, and histology

A
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15
Q

Flow cytometry samples

A

Cells must be in a liquid suspension (blood or BM in purple top, cavity fluid, tissue aspirate in liquid media)
Cells must be alive (ship on ice)
DONT SEND: glass slides, frozen, formalin-fixed

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16
Q

What is the approach to a case of lymphadenopathy?

A
17
Q

Describe the principles of PARR

A

Looks for clonality at DNA level
Polyclonal = antigenic stimulation
Monoclonal = neoplasia
Differentiates B cell vs T cell neoplasm
Major use: neoplastic v non-neoplastic
Examines the size of the Ig and t cell receptor genes

18
Q

Evaluation of Neutrophils

A

Count
Measure expression of surface proteins associated with migration out of vessels (CD18)
Neutrophil function assay

19
Q

Evaluation fo B cells

A

Flow cytometry (CD21+)
Measure Ab levels (radial immunodiffusion assay, serum protein electrophoresis/immunofixation)

20
Q

Evaluation of T cells

A

Quantify T cell subsets by flow cytometry

Bioanalytical Pathology (29 decks)

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