Detecting Proteins Using Antibodies

Question 1

Immunoassay Techniques

Answer 1

Used to detect and identify specific proteins

Question 2

Monoclonal antibodies

Answer 2

stocks of antibodies with the same specificity.

Question 3

label

Answer 3

An antibody specific to the protein antigen is linked to a chemical ‘label’

Question 4

Western blotting

Answer 4

The separated proteins from the gel are blotted onto a solid medium

Question 5

The proteins can be identified using…

Answer 5

specific antibodies that have reporter enzymes
attached

Question 6

Immunoassay

Answer 6

COVID-19 (monoclonal) antibodies are attached to an assay plate

Sample of blood infected with COVID-19 antigens is added to assay plate

Antigens in infected blood bind to antibodies on the plate

Plate is washed with buffer

Monoclonal antibodies and an attached reporter enzymes specific to COVID-19 are added to assay
plate

Antibodies (with attached reporter enzymes) bind to COVID-19

Plate is washed with buffer

A colourless substrate is added to the plate

Reporter enzyme degrades this substrate to produce a coloured product

Coloured product indicates patient is positive for COVID-19