DEFINITION OF TERMS Flashcards
A branch of science that studies the interaction between electromagnetic radiation and matter.
Spectroscopy
A procedure by which solutes are separated by a differential migration process in a system consisting of 2 or more phases
Chromatography
The SP is a solid on which the sample is adsorbed while the MP may be a liquid or a gas
Adsorption chromatography
- Based on partition between 2 immiscible solvents
- Both SP and MP are in liquid form
Partition chromatography
- SP: water molecules bound to the cellulose of the filter paper
- MP: nonpolar or hydrophobic solvent
Paper chromatography
Used for the separation of charged molecules (ion) based their binding to fixed charges on a support
Ion-exchange chromatography
SP: employs either a cationic or an anionic exchanger which is capable of exchanging counter ions in the surrounding
medium in a reversible process
Ion-exchange chromatography
Molecules are separated according to their size by their ability to penetrate a sieve-like structure
Permeation chromatography
Ex: Size Exclusion Chromatography
Utilized highly specific interactions between one kind of solute molecule and a second molecule covalently attached to the SP
Affinity chromatography
Ex: protein affinity chromatography
Give the temperature equivalence
- Very dull red 500-550
- Dull red 550-700
- Bright red 800-1000
- Yellow red 1000-1200
- White 1200-1600
USP Method I for Iodine value
Hanus Method - Iodobromide TS
USP Method II for Iodine value
Wijs Method- Iodochloride TS
Unofficial Method for Iodine value
Hubl Method - HgCl2 + I2
Conversion of Organic N into NH4+ by adding H2SO4
Digestion
Conversion of NH4+ into ammonia
Distillation