deck_582382 Flashcards
Give three structural similarities between Ca2+ ion channels and Na+ ion channels
- 4 homogenous repeats which are linked together* Each repeat has 6 transmembranous domains one of which is voltage sensitive* The four repeats aggregate together to form a functional channel
Name a main location where Ca2+ ion channels found
Nerve terminals
Why does the internal concentration of Ca2+ change so significantly when Ca2+ ion channels open?
Because the concentration of Ca2+ inside is so low, the Ca2+ influx through Ca2+ channels is relatively very high.
Give the sequence of events which causes neutransmitter release at nerve terminals
- Depolarization2. Voltage-gated Ca2+ channels open3. Ca2+ influx > down concentration gradient.
Name the TYPE, BLOCKER and PRIMARY LOCATION of each isoform of Ca2+ ion channel a1C,D,S
Type - LBlocker - DHPPrimary location - All muscles, neurones, lungs
Name the TYPE, BLOCKER and PRIMARY LOCATION of each isoform of Ca2+ ion channel a1B
Type - NBlocker - w-CTx-GVIAPrimary location - Neurones
Name the TYPE, BLOCKER and PRIMARY LOCATION of each isoform of Ca2+ ion channel a1A
Type - P/Q Blocker - w-Aga-IVAPrimary location - Neurones
Name the TYPE, BLOCKER and PRIMARY LOCATION of each isoform of Ca2+ ion channel a1E
Type - RBlocker - Ni2+Primary location - Unknown (neurones, heart)?
Name the TYPE, BLOCKER and PRIMARY LOCATION of each isoform of Ca2+ ion channel a1G,H,I
Type - TBlocker - Ni2+Primary location - Neurones, heart
What is the most common type of Ca2+ channel?
L type
What is Nifedipine, and what does it do?
Nifedipine (a DHP) is used to control hypertension as it can act on vascular smooth muscle and prevent their constriction by blocking the L type Ca2+ channel. Ca2+ needed for contraction.
What is the process via which neurotransmitters are released called?
Exocytosis
In motor neurone terminals, what does increase in Ca2+ activate?
A group of proteins associated with the vesicle to promote exocytosis of ACh.
Describe the process of events underlying fast synaptic transmission.
- Depolarisation > voltage-gated ion channels open> Ca2+ influx* Ca2+ binds to synaptotagmin* Vesicle brought close to membrane* Snare complex (vesicle + protein) make a fusion pore* Transmitter released through this pore
What is special about the location of Ca2+ in nerve terminals?
- Ca2+ channels located close to vesicle release sites
What happens to vesicle contents once released from the synapse?
- ACh binds to nicotinic ACh receptor on the post-junctional membrane to produce an end-plate potential* Depolarisation, raises muscle above threshold so that an action potential is produced in the muscle membrane
Give six properties of nicotinic acetycholine receptors
- Intrinsic pore2. Activated by binding of a chemical ligand (an atom, ion, molecule or radical which binds to a central atom forming a complex) to a site on the channel molecule e.g. ACh to site on nicotinic receptor3. Activation causes the pore to open4. Allows influx of Na+ into the cell5. Acetylcholinesterase breaks down ACh rapidly6. No binding of ACh > pore closed
Name a competitive blocker of nicotinic receptors
Tubocurarine
How does tubocurarine work?
Competitive blocker * d-tubocurarine (d-TC) binds to ligand site on receptor* ACh cannot bind > channel remains closed* Can be outcompeted by increasing ACh
Name a depolarising blocker
succinylcholine