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deck_5661103 Flashcards

1
Q

What is a condensation reaction?

A

When two molecules are joined together with the removal of water

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2
Q

What is a hydrolysis reaction?

A

When a molecule is split into two smaller molecules with the addition of water

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3
Q

How do almost all condensation reactions happen?

A

When two -OH groups react together-this reaction involves the breaking and formation of covalent bonds

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4
Q

In condensation reactions, what molecules are joined together?

A

Monomers

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5
Q

What if formed when lots of monomers join together?

A

Polymers

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6
Q

What do carbohydrates contain?

A

Carbon, hydrogen and oxygen. The monomer is a monosaccharide and the polymer is a polusaccharide

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7
Q

What do proteins contain?

A

Carbon, hydrogen, oxygen, nitrogen and sulfur. The monomer is amino acids and the polymer is polypeptides and proteins

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8
Q

What do nucleic acids contain?

A

Carbon, hydrogen, oxygen, nitrogen and phosphorus. The monomer is nucleotides and the polymer is DNA and RNA

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9
Q

What does water consist of?

A

Two hydrogen atoms covalently bonded to one oxygen atom, however the oxygen atom has a greater number of protons in it’s nucleus which exerts a stronger attraction for the shared electrons, so the oxygen atom becomes slightly negative and the hydrogen become slightly positive, the molecule is polar

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10
Q

What is a hydrogen bond?

A

A weak interaction which happens wherever molecules contain a slightly negatively charged atom bonded to a slightly positively charged hydrogen atom. It is weaker than a covalent bond however some polymers have thousands of hydrogen bonds that form between chains of monomers, helping stabilise the structure of some biological molecules

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11
Q

How are hydrogen bonds represented in diagrams?

A

With a dotted line

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12
Q

What are the properties of water?

A

Latent heat of evaporation, density, transparent, dipolar, surface tension, incompressibility, temperature, specific heat, viscosity, solvent, metabolic, capillarity and state

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13
Q

What is latent heat of evaporation?

A

Water molecules absorb heat energy from surfaces as they evaporate, cooling them down eg sweating and transpiration

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14
Q

What is density?

A

Water supports aquatic organisms. Ice floats on water allowing organisms to survive below it in liquid. Polar bears live in an environment of floating ice planks

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15
Q

What is transparent?

A

Allows the transmission of light eg photosynthesis in aquatic organisms

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16
Q

What is dipolar?

A

Has positive and negative charges

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17
Q

What is surface tension?

A

Water has a strong surface film allowing small organisms to be supported on or in it

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18
Q

What is incompressibility?

A

Provides a hydrostatic skeleton with a surrounding strong wall provides support and protection eg earthworm body cavity, human abdomen and plant cells

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19
Q

What is temperature?

A

Carries away heat energy when it evaporates from a surface. This cools the surface and helps to lower the temperature

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20
Q

What is specific heat?

A

High specific heat so gains and loses heat slowly, good for temperature control of aquatic environments

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21
Q

What is viscosity?

A

Allows free flow for transport of materials inside and outside the organisms

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22
Q

What is solvent?

A

Universal solvent to dissolve many substances

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23
Q

What is metabolic?

A

Water takes part as a reactant in some chemical processes-hydrolysis and photosynthesis

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24
Q

What is capillarity?

A

Water rises in small tubes against gravity because polar molecules are attracted to themselves and to surfaces, useful in plants eg xylem and phloem

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25
Q

What is state?

A

Solid at 0ºc and vapour above 100ºc provides a wide temperature range as a liquid

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26
Q

What are ‘hydrated carbon’?

A

For every carbon there are two hydrogen atoms and one oxygen atom. Carbohydrates are hydrated carbon

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27
Q

What are the functions of carbohydrates?

A

They act as a source of energy (glucose), as a store of energy (starch/glycogen) and as structural units (cellulose in plants and chitin in insects). Some are also part of other molecules such as nucleic acids and glycolipids

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28
Q

What are the three main groups of carbohydrates?

A

Monosaccharides, disaccharides and polysaccharides (common monosaccharides and disaccharides all have names ending in -ose)

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29
Q

What are monosaccharides?

A

They are the simplest carbohydrates and are particularly important in living things as a source of energy

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30
Q

How are monosaccharides well suited to their role?

A

Because they have a large number of carbon-hydrogen bonds

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31
Q

What are the properties of monosaccharides?

A

They are sugars, which taste sweet, are soluble in water and are insoluble in non-polar solvents

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32
Q

What is the structure of monosaccharides?

A

They can exist as straight chains or in ring/cyclic forms. They have a backbone of single bonded carbon atoms, with one double-bonded to an oxygen atom to form a carbonyl group

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33
Q

What are the different sugars with different numbers of carbon atoms?

A

Hexose sugars have six carbon atoms, pentose sugars have five carbon atoms and triose sugars have three carbon atoms

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34
Q

What is an example of a monosaccharide hexose sugar?

A

Glucose, these types are the monomers of more complex carbohydrates, and they bond together to form disaccharides or polysaccharides

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35
Q

What are triose and tetrose sugars like in solution?

A

They exist as straight chains

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36
Q

What form are pentoses and hexoses more likely to be found as?

A

Ring or cyclic form

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37
Q

What are isomers?

A

Molecules with the same formula, but whose atoms are arranged differently, glucose in both forms can exist as a number of different isomers

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38
Q

How are isomers formed in straight chains?

A

The -H and -OH can be reversed

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39
Q

How is the ring shape formed?

A

When the oxygen attached to carbon 5 bonds to carbon 1. Because the -OH and -H on carbon 1 can be above or below, there are two isomers (a- and b-glucose), the small difference seems insignificant but becomes very important when glucose polymerises into starch or cellulose

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40
Q

What are the properties of disaccharides?

A

They are sweet and soluble

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41
Q

What are the most common disaccharides?

A

Maltose and lactose with are reducing sugars, and sucrose which is a non reducing sugar

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42
Q

How are disaccharides made?

A

When two monosaccharides join together

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43
Q

What is a-glucose plus a-glucose?

A

Maltose

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44
Q

What is a-glucose plus fructose?

A

Sucrose

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45
Q

What is b-glucose plus a-glucose?

A

Lactose

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46
Q

What is b-glucose plus b-glucose?

A

Cellobiose

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47
Q

What happens when the monosaccharides join?

A

A condensation reaction occurs to form a glycosidic bond. Two hydroxyl groups line up next to each other, from which a water molecule is removed, leaving an oxygen atom acting as a link between the two monosaccharide units

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48
Q

How are disaccharides broken into monosaccharides?

A

By a hydrolysis reaction which requires the addition of water. The water provides a hydroxyl group -OH and a hydrogen -H which help the glycosidic bond to break eg cellobiose is obtained by the hydrolysis of cellulose

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49
Q

Explain a-glucose

A

All -H’s are above the C, except for at C3. Molecular formula is C6H12O6. It is an energy source/component of starch/glycogen which acts as energy stores. It is a hexose sugar

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50
Q

Explain b-glucose

A

-OH are above at carob 1 and 3. Molecular formula is C6H12O6. It is an energy source/component of cellulose with provides structural support in plant cell walls. It is a hexose sugar

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51
Q

Explain ribose

A

Molecular formula is C5H10O5. It is a component of RNA, ATP and NAD. It is a pentose sugar

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52
Q

Explain deoxyribose

A

Molecular formula is C5H10O4. It is a component of DNA. It is a pentose sugar

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53
Q

What are polysaccharides?

A

Polymers of monosaccharides made of hundreds/thousands of monosaccharides bonded together

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54
Q

What are homopolysaccharides?

A

Polysaccharides made solely of one kind of monosaccharide

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55
Q

What are heteropolysaccharides?

A

Polysaccharides made up of more than one monomer

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56
Q

What is an example of a homopolysaccharide?

A

Starch

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57
Q

What is an example of a heteropolysaccharide?

A

Hyaluronic acid

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58
Q

Why is glucose a source of energy?

A

It is a reactant in respiration. The energy released is used to make ATP, which is the energy currency of the cell

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59
Q

How can you create a store of energy?

A

By joining lots of glucose molecules together into polysaccharides, this is what living things do

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60
Q

How do plants store energy?

A

As starch in chloroplasts and in membrane bound starch grains

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61
Q

How do humans store energy?

A

As glycogen in cells of the muscles and liver

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62
Q

Why are glycogen and starch (amylose and amylopectin) good energy stores?

A

They are compact so they don’t occupy a large amount of space. They both occur in dense granules within the cell

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63
Q

Why do polysaccharides hold glucose molecules in chains?

A

So they can be easily ‘snipped off’ from the end of the chain by hydrolysis when required for respiration. Hydrolysis reactions are always catalysed by enzymes

64
Q

What are amylose chains like?

A

Unbranched

65
Q

What are amylopectin and glycogen chains like?

A

Branched

66
Q

What are branched chains?

A

They are more compact but also offer the chance for lots of glucose molecules to be snipped off by hydrolysis at the same time, when lots of energy is required quickly

67
Q

What is amylase?

A

The enzyme responsible for hydrolysing 1-4 glycosidic linkages. A 1-4 glycosidic linkage is one between carbon 1 and of one glucose and carbon 4 of the other

68
Q

Why is it good that polysaccharides are less soluble in water than monosaccharides?

A

If many glucose molecules did dissolve in the cytoplasm, the water potential would reduce, and excess water would diffuse in, disrupting the normal workings of the cell

69
Q

Why are polysaccharides less soluble?

A

Because of their size, and also because regions which could hydrogen bond with water are hidden away inside the molecule

70
Q

What happens if the amylose molecule forms a double helix?

A

It presents a hydrophobic external surface in contact with the surrounding surface

71
Q

What is amylose?

A

(In plants) The molecule is a long chain of a-glucose molecules. Like maltose, it has glycosidic bonds between carbons 1 and 4

72
Q

What is the detailed structure of amylose?

A

Coils into a spiral shape, with hydrogen bonds holding the spiral in place. Hydroxyl groups on carbon 2 are situated on the inside of the coil, making the molecule less soluble and allowing hydrogen bonds to form to maintain the coil’s structure

73
Q

What is amylopectin?

A

(In pants) Has glycosidic bonds between carbons 1 and 4 but also has branches formed by glycosidic bonds between carbon 1 and 6

74
Q

What is the detailed structure of amylopectin?

A

Coils into a spiral shape, held together with hydrogen bonds, but with branches emerging from the spiral

75
Q

What is glycogen?

A

(In animals) Has glycosidic bonds between carbon 1 and 4, and branches formed by glycosidic bonds between carbon 1 and 6

76
Q

What is the detailed structure of glycogen?

A

The 1-4 bonded chains tend to be smaller than in amylopectin, so glycogen has less tendency to coil, however, it does have more branches, which makes it more compact. And it is easier to remove monomer units as there are more ends

77
Q

What is cellulose?

A

Found in plants, form cell walls, tough, insoluble, fibrous, homopolysaccharide of b-glucose bonded through condensation reaction forming glycosidic bonds

78
Q

What form are cellulose chains?

A

Straight and lie side by side. This different structure is a direct result of bonding

79
Q

What happens to the hydrogen and hydroxyl group on carbon 1 in b-glucose?

A

They are inverted, meaning every other b-glucose molecule in the chain is rotated by 180º, and the b-1-4 glycosidic bond help to prevent the chain spiralling

80
Q

What do the hydrogen bonds do?

A

They are between the rotated b-molecules in each chain and gives the chain additional strength and stops it spiralling

81
Q

What does the hydroxyl group on carbon 2 sticking out enable?

A

Hydrogen bonds to be formed between chains

82
Q

What happens when 60-70 cellulose chains are bound together in this way?

A

They form microfibrils, which bundle together into macrofibrils containing up to 400 microfibrils which are embedded in pectins to form plant cell walls. Macrofibrils run in all directions criss-crossing the wall for extra strength

83
Q

What is the structure of cellulose chains from biggest to smallest?

A

Cellulose fibres, macrofibrils, microfibrils, chains of cellulose molecules

84
Q

Why is cellulose an excellent material for plant cell walls?

A

Micro and macrofibrils have very high tensile strength due to glycosidic bonds and hydrogen bonds between chains. Macrofibrils criss cross in all directions for extra strength. It is difficult to digest cellulose due to glycosidic bonds which are less easy to break and most animals don’t have the enzyme to catalyse the reaction

85
Q

What are the key features that help the plant cell wall to do it’s job?

A

Plants don’t have a rigid skeleton so each cell needs strength to support whole plant, space between macrofibrils for water/mineral ions to pass in/out of the cell making the wall fully permeable, wall has high tensile strength preventing cells from bursting, macrofibril structure can be reinforced with other substances for extra support eg Lignin in xylem vessles

86
Q

How has the structural strength of cellulose been exploited by humans?

A

Cotton is 90% cellulose. Cellophane and celluloid are derived from cellulose. Cellulose is a main component in paper. Rayon is produced from cellulose

87
Q

What are bacterial cell walls?

A

They aren’t made of cellulose. The whole structure surrounding the cell is called a peptidoglycan made from long polysaccharide chains that lie in parallel, cross linked by short peptide chains made of amino acids

88
Q

What are exoskeletons?

A

Insect and crustacean exoskeletons are made of chitin. Differs from cellulose as has a acetylamino group instead of a hydroxyl group on carbon 2. It forms crosslinks between long parallel chains of acetylglucosamine, in a similar way to cellulose

89
Q

What are lipids?

A

Contain large amounts of carbon and hydrogen, and smaller amounts of oxygen. Insoluble in water as they aren’t polar so don’t attract water molecules. Dissolve in alcohol

90
Q

What are the three most important lipids in living things?

A

Triglycerides, phospholipids and steroids. They aren’t polymers but do have different components bounded together, they are examples of macromolecules

91
Q

What are triglycerides made up of?

A

Glycerol and fatty acids. Many different types of fatty acid and many can be made in human bodies but some have to be ingested ‘complete’, called essential fatty acids

92
Q

What is glycerol?

A

It has three carbon atoms, it is an alcohol which means it has free -OH groups. There are three –OH groups which are important to the structure of triglycerides

93
Q

What are fatty acids?

A

They have a carboxyl group on one end, attached to a hydrocarbon tail made of only carbon and hydrogen atoms. This can be 2-20 carbons long. The carboxyl group ionises into H+ and -COO- group. This structure is therefore an acid as it can produce free H+ ions

94
Q

What are saturated fatty acids?

A

No c=c bonds in the molecule

95
Q

What are unsaturated fatty acids?

A

Contain a double bond between two of the carbon atoms so fewer hydrogen atoms can be bonded to the molecule

96
Q

What does a single c=c bond do?

A

Makes a fatty acid monounsaturated

97
Q

What does more than one c=c bond do?

A

Makes a fatty acid polyunsaturated

98
Q

What happens to the hydrocarbon chain if there is more than one c=c bond?

A

It changes shape, giving it a kink where the double bond is, which push the molecules apart slightly making them more fluid

99
Q

What sort of fatty acids do animal lipids contain?

A

Lots off saturated fatty acids which are often solid at 20ºc. Unsaturated fatty acids have a lower melting point

100
Q

What is an ester bond?

A

When a condensation reactions bonds together one glycerol molecule and three fatty acids

101
Q

What are the functions of triglycerides?

A

Energy source, energy store, insulation, buoyancy and protection

102
Q

How are triglycerides used as an energy source?

A

can be broken down in respiration to release energy and generate ATP

103
Q

How are triglycerides an energy store?

A

Insoluble in water so they can be stored without affecting the water potential of the cell

104
Q

How are triglycerides used for insulation?

A

Adipose tissue is a storage location for lipid in whales, acting as a large insulator. Lipid in nerve cells acts as an electrical insulator. Animals preparing for hibernation store extra fat

105
Q

How are triglycerides used for buoyancy?

A

Fat is less dense than water, it is used by aquatic mammals to help them stay afloat

106
Q

How are triglycerides used for protection?

A

Humans have fat around delicate organs such as the kidneys, to act as a shock absorber. The peptidoglycan cell wall of some bacteria is covered in a lipid-rich outer coat

107
Q

What is the structure of phospholipids?

A

Same structure as triglycerides except one of the fatty acids is replaced by a phosphate group.

108
Q

What are phospholipids behaviour in water?

A

The phosphate group has a negative charge making it polar but the fatty acid tails are non polar. Hydrophilic head and hydrophobic tail, making the phospholipid amphipathic

109
Q

What different properties do amphipathic phospholipids have in water?

A

They may form a layer on the surface of the water with heads in the water and tails sticking up out of the water. They may also form micelles (tiny balls with the tails tucked inside and heads pointing outwards into the water)

110
Q

What are amphipathic phospholipids good at forming membranes around cells and organelles?

A

Inside and outside a cell membrane is an aqueous solution, the phospholipids form a bilayer with tails in and heads out. The individual phospholipids can move but the tails are never exposed, giving the membrane some stability

111
Q

What does it mean, that the membrane is selectively (partially) permeable?

A

It is only possible for small and non-polar molecules to move through the tails in the bilayer, such as oxygen and carbon dioxide, letting the membrane control what goes in and out of the cell, and keeps it functioning properly

112
Q

What is cholesterol?

A

A steroid alcohol or type of lipid which is not made from glycerol or fatty acids. It consists of four carbon-based rings. it is small and hydrophobic so can sit in the middle of the phospholipid bilayer and regulate the fluidity of the membrane

113
Q

Where is cholesterol found?

A

Mainly in the liver in animal cells, plants however also have a cholesterol derivative in their membranes which is different due to a double bond between carbon 22 and 23

114
Q

What are some hormones made from cholesterol?

A

The steroid hormones testosterone, oestrogen and vitamin D. They are small and hydrophobic so can pass through the hydrophobic part of the membranes, these hormones are also abundant in plants, and on ingestion and absorption can be converted into animal hormones

115
Q

What are proteins?

A

Large polymers comprised of long chains of amino acids

116
Q

What are proteins functions?

A

Form structural components of animals in particular eg muscles. Their tendency to adopt specific shapes make proteins important as enzymes, antibodies and some hormones. Membranes have protein constituents that act as carriers and pores for active transport across the membrane and facilitated diffusion

117
Q

How do animals get amino acids to make proteins?

A

They can make some but others must be ingested (essential amino acids)

118
Q

How do plants get amino acids needed to make proteins?

A

They can make all the amino acids they need but only if they can access fixed nitrogen such as nitrate

119
Q

What does each amino acid contain?

A

The elements carbon, hydrogen, oxygen and nitrogen. Some amino acids also contain sulfur

120
Q

How many amino acids are there?

A

Over 500, but only 20 of them are proteinogenic (found in proteins)

121
Q

What does each amino acid chain have?

A

An amino group (-NH2) at one end, and a carboxyl (-COOH) group at the other end. There is also an R group which is different in each amino acid

122
Q

What do names of almost all amino acids end in?

A

-ine, except for those which have an acidic R group

123
Q

How can R groups vary?

A

By size, charge, polarity and whether they are hydrophobic or hydrophilic

124
Q

What is a peptide bond?

A

A bond formed when two amino acids are joined by a condensation reaction. Enzymes catalyse these reactions. Protease enzymes in intestines break down peptide bonds during digestion. They also break down protein hormones so that their effects are not permanent

125
Q

What is a primary structure?

A

The sequence of amino acids found in a molecule

126
Q

What is a secondary structure?

A

The coiling or folding of an amino acid chain, which arises often as a result of hydrogen bond formation between different parts of the chain. The main forms of secondary structure are the helix and the pleated sheet

127
Q

What is a tertiary structure?

A

The overall three-dimensional shape of a protein molecule. Its shape arises due to interactions including hydrogen bonding, disulphide bridges , ionic bonds and hydrophobic interactions

128
Q

What is a quaternary structure?

A

Protein structure where a protein consists of more than one polypeptide chain eg insulin has a quaternary structure

129
Q

What are fibrous proteins?

A

Have regular, repetitive sequences of amino acids, and are usually insoluble in water. These features enable them to form fibres which tend to have a structural function

130
Q

What are examples of fibrous proteins?

A

Keratin, elastin and collagen

131
Q

What is keratin?

A

Rich in cysteine with lots of disulphide bridges between it’s polypeptide chains. Also hydrogen bonding makes the molecule very strong. Found wherever body parts need strength and hardness eg nails, hairs, claws, hooves, horns, scales, fur and feathers. It provides mechanical protection and an impermeable barrier to infection as it’s waterproof

132
Q

What is elastin?

A

Cross-linking/coiling make structure strong. Found in living things where they need to stretch or adapt their shape as part of life processes eg skin stretched around bones and muscles, elastin in lungs lets them inflate and deflate, and helps blood vessels to stretch and recoil

133
Q

What is collagen?

A

Provides mechanical strength. In the artery walls it prevents arteries bursting when withstanding high pressure from blood being pumped by the heart, and tendons are made of collagen and connect muscles to bones allowing them to pull on bonesq

134
Q

What are globular proteins?

A

Tend to recoil into an almost spherical shape. Any hydrophobic R groups are turned inwards towards centre of molecule while hydrophilic groups are on the outside. Makes proteins water soluble as water molecules can easily cluster round and bind to them. Have specific shapes which help their roles as enzymes, hormones and haemoglobin

135
Q

What are examples of globular proteins?

A

Insulin, haemoglobin and pepsin

136
Q

What is insulin?

A

Made of two polypeptide chains, A chain beings with alpha helix and B chain ends with a beta pleat. Both fold into tertiary structure and join by disulphate links. Amino acids with hydrophilic R group are on outside making it water soluble. Insulin binds to glycoprotein receptors on outside of muscle and fat cells to increase uptake of glucose from blood and increase rate of consumption of glucose

137
Q

What is haemoglobin?

A

Consists of four polypeptide subunits, two are a-chains and two are b-chains. It is a water-soluble globular protein

138
Q

What is pepsin?

A

An enzyme that digests protein in the stomach. The enzyme is made of a single polypeptide chain of 327 amino acids, but folds into a symmetrical tertiary structure

139
Q

What are examples of cations?

A

Calcium, Sodium, Potassium, Hydrogen and Ammonium

140
Q

What is calcium?

A

Ca2+ Increases rigidity of bone, teeth and cartilage. Component of exoskeleton. Blood clotting and muscle contraction. Activator for enzymes. Regulates permeability of cell membranes. Cell wall development in plants

141
Q

What is sodium?

A

Na+ Regulation of osmotic pressure, water levels and pH. Absorption of carbohydrates in intestine and water in kidney. Nervous transmission and muscle contraction. Constituent of vacuole

142
Q

What is potassium?

A

K+ Control water levels and pH. Assists active transport across cell membrane. Synthesis of glycogen/protein and breakdown of glucose. Generates healthy leaves and flowers. Nervous transmission and muscle contraction. Component of vacuole

143
Q

What is hydrogen?

A

H+ Photosynthesis/respiration. Transport of oxygen and carbon dioxide in blood. Regulation of blood pH

144
Q

What is ammonium?

A

NH4+ Component of amino acids/proteins/vitamins/chlorophyll, some hormones, essential component of nucleic acids, maintenance of pH inhuman body, component of nitrogen cycle

145
Q

What are examples of anions

A

Nitrate, hydrogencarbonate, chloride, phosphate and hydroxide

146
Q

What is nitrate?

A

NO3- Component of amino acids/proteins/vitamins/chlorophyll. Component of nucleic acids. Some hormones. Component of nitrogen cycle

147
Q

What is hydrogencarbonate?

A

HCO3- Regulation of blood pH. Transport of carbon dioxide into and out of blood

148
Q

What is chloride?

A

Cl- Production of urine in kidney/maintaining water balance. Transport of carbon dioxide into and out of blood. Regulation of haemoglobin. Regulation of blood pH. Production of hydrochloric acid in stomach

149
Q

What is phosphate?

A

PO43- Increases rigidity in bone, teeth and cartilage. Component of exoskeleton. Component of phospholipids, ATP, nucleic acids, enzymes. Regulation of blood pH. Root growth in plants

150
Q

What is hydroxide?

A

OH- Regulation of blood pH

151
Q

What is the test for starch?

A

Add iodine solution to a sample. If starch is present the colour changes from yellow/brown to blue/black

152
Q

What is the test for reducing sugars?

A

Add glucose, water and Benedict’s solution in boiling water. Orange-red participate indicates a reducing sugar is present

153
Q

What is the test for non-reducing sugars?

A

If reducing test is negative, boil with HCL, cool and neutralise with sodium carbonate solution then repeat Benedict’s test. On second test it should turn blue to orange/red

154
Q

What is the test for proteins?

A

Add biuret reagent. If present then it will turn blue to lilac

155
Q

What is the test for lipids?

A

Add ethanol, then pour into another test tube of water. White emulsion forms near top of water if there are lipids

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