Deck 5- Transforming Bacteria (for Insulin)

Question 1

First, a ________ ________ is used to cut the human insulin gene out of the human cell’s DNA.

Answer 1

Restriction enzyme

Question 2

Second, using the same restriction enzyme, a _______ ________ is taken out of the bacteria and cut open, producing matching sticky ends.

Answer 2

Bacterial plasmid

Question 3

Third, the human insulin gene is added to the bacterial plasmids in a Petri dish because of the matching _______ ______.

Answer 3

Sticky ends

Question 4

Fourth, because the DNA in the plasmid contains bacteria and human DNA, it is now called ______ ______.

Answer 4

Recombinant DNA

Question 5

Fifth, the recombinant plasmids are mixed with the bacteria, and some bacteria take up ______ ______, which is a transformation.

Answer 5

Recombinant plasmid

Question 6

Sixth, the recombinant bacteria can then ________ asexually.

Answer 6

Reproduce

Question 7

Recombinant bacteria can make many offspring each with the _______ _______ gene and can produce lots of insulin (only need to do once).

Answer 7

Human insulin