Deck Flashcards
Cyclin
Proteins that start certain steps of mitosis
Cyclin-dependent kinase
An enzyme that catalyzes the transfer of a phosphate group from ATP to a specified molecule.
How is a microarray made?
Printing DNA using an arrayer
What do microarrays detect?
Over or underexpression of genes in cancer vs healthy cells
Green spot in microarray
Less active in cancer
Red spot in microarray
More active in cancer
cDNA in microarrays
Copy/complementary DNA, hybridizes with sample
Why do we use cDNA instead of RNA?
It’s more stable and less prone to degradation
Ultrasounds
Soundwaves emitted from transducer, beams bounce back to collect data (3.5-7 MHz)
X-ray
2D images, structures containing air will be black. Muscle/fat/fluid all gray, uses radiation
Pros of X rays
Noninvasive, quick and painless, cheap
Cons of X rays
2D images, cancer risk, structural info
CT scan
Combines X rays from multiple angles
Pros of CT scans
Noninvasive, accurate, quick and painless, full body, can be performed with implant
Cons of CT scans
Radiation, uses contrast media, structural not functional