Decalcification Flashcards
decalcification temperature
18-30deg C
an excellent bone decalcifier for EM and enzyme histochemistry
EDTA
EDTA can inactivate _
remedy:
alkaline phosphatase
remedy: add magnesium chloride
most rapid method of decalcification due to electric current calcium is removed
electrolytic method
method of testing for the completeness of decalcification that uses calcium carbonate
bubble test
bubble test uses
calcium carbonate
If after decalcification, the tissue is still hard use
Molliflex-Perenyi’s fluid
(double purpose)
i. Decalcifying agent
ii. Tissue softener
perenyi’s fluid
decalcifying acid used for surface decalcification of blocks
hydrochloric acid
- most rapid nitric containing decalcifying agent
Phloroglucin Nitric Acid
t/f
Osteoporotic specimen has low calcium content = shorter decalcification time
true
duration of decalcification thus ion exchange resin
1-14 days
Methods to Measure Extent of Decalcification
- Mechanical / Physical
- X-ray / Radiologic
- Chemical
- Bubble Test
Methods in Decalcification
- Use of acid
- Use of decalcifying agent
- Ion Exchange Resin
- Electrolytic Method
most commonly used decalcifying agent
5-10% Nitric Acid
