Clearing / Dealcoholization

Question 1

can be used as xylene & benzene substitute

Answer 1

toulene

Question 2

benzene can damage _ and may cause _ on prolong use

Answer 2

bone marrow; aplastic anemia

Question 3

routinely used clearing agent but not for nervous tissues and lymph nodes

Answer 3

xylene

Question 4

can become milky after placing an incompletely dehydrated tissue in it

Answer 4

xylene

Question 5

can become milky because of prolonged storage

Answer 5

cedarwood oil

Question 6

• clearing agent for CNS tissues & cytological studies,
• an extremely slow clearing agent

Answer 6

cedarwood oil

Question 7

toxic to liver, recommended for skin, fiber & decalcified tissues

Answer 7

chloroform

Question 8

clearing agent that will not make tissues transparent
- clearing agent that can attack rubber seal used in vacuum impregnation

Answer 8

chloroform

Question 9

same property as chlorofotm

Answer 9

carbon tetrachloride

Question 10

• for clearing insects, embryos & other delicate specimens

Answer 10

aniline oil

Question 11

when used, tissues become adulterated

Answer 11

clove oil

Question 12

• clearing agent obtain from citrus fruit
• with faint pleasant odor

Answer 12

limonene

Question 13

recommended for eyes specimens

Answer 13

terpineol

Question 14

chloroform & cedarwood oil

Answer 14

• clearing agents that can be used for dense tissues like uterus
• clearing agents difficult to remove from tissues

Question 15

Clearing agent easily removed from tissues

Answer 15

benzene and xylene

Question 16

Clearing agents for double embedding (SLOW ACTING AGENTS)

Answer 16

1. Methyl salicylate
2. Methyl benzoate
3. Amyl acetate