cytoskeleton Flashcards

1
Q

3 fibers that make up eukaryotic cytoskeleton

A

microfilaments
intermediate filaments
microtubules

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2
Q

microtubules structure

A

comprised of alpha and beta tubulin dimers
hollow tube shape

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3
Q

microtubules function

A

cell shape movements
movement of chromosomes during cell division
used by cell to move organelles, proteins, viruses
has specialized motor proteins

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4
Q

microtubules dynamic instability

A

constantly switching between growth and shortening at microtubules ends

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5
Q

treadmilling microtubules

A

there are areas that dimmers were more likely to fall off or more likely to join the microtubules at
dimers that fell off came back and joined the microtubule at the end where it was more likely to join back

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6
Q

what eukaryotic motility structures do the microtubules comprise

A

flagella
cilia

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7
Q

specialized motor proteins of microtubules

A

kinesin
dynein

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8
Q

kinesin

A

is the motor protein that moves towards the plus end
end of microtubule where polymerization occurs at a higher rate (more dimers being added than removed)

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9
Q

dynein

A

the motor protein that moves towards the minus end
minus end: end of microtubule where dimers fall off at a higher rate
what allows movement of motor proteins

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10
Q

what allows movement of motor proteins

A

ATP hydrolysis changes the shape of the proteins, allowing them to move along the microtubule

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11
Q

how do flagella and cilia differ

A

in terms of movement and length of the structures
flagella
rotational movement
cilia
whip like motion

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12
Q

MTOCs

A

microtubules are organized in cells by MTOCs
barrel shaped structures made of 9 sets of triplet microtubules
usually found very close to the nucleus
surrounded by an ill-defined pericentriolar matrix

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13
Q

microfilaments - composition

A

comprised out of actin monomers
f actin are actin that are bound to microfilaments
g actin are floating actin

actin bind only when carrying ATP

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14
Q

microfilaments function

A

cell shape
cell movement
plus and minus ends of micro filament depend on rate of addition and falling off of actin
polymerization –> gactin turn into factin
depolymerization –> factin –> gactin

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15
Q

treadmilling with microfilaments

A

governed by ATP hydrolysis
actin with bound ATP join the microfilament
actin with bound ADP fall off

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16
Q

motor protein
microfilaments

A

myosin
use ATP to move in both directions

17
Q

different microfilaments and their role in cell shape

A

stress fibers: bundles of actin filaments that help maintain skin cell shape and keeps cells elongated

focal adhesions
places where stress fibers attach to the plasma membrane and anchor cells to the substrate or to adjacent cells

microvilli
protrusions filled with stable actin bundles projecting from the cell surface

18
Q

intermediate filaments

A

many different intermediate filament proteins exist but they all build similar cable like filaments
very stable networks
provide structural support
lamins
not dynamic

19
Q

outside of the plasma membrane
plant cells are encased in

A

cellulose which acts as a cell wall

primary cell wall and a secondary cell wall laid on when the cell stops expansion

two cell walls stuck together by middle lamella

20
Q

plant cells linked to neighboring cells through

A

pores that link plasma membranes called plasmodesmata

21
Q

animal cells dont have a cell wall
how does this change things for them

A

less rigid
anchor themselves to the ECM
matrix of glycoproteins and carb-containing cells

22
Q

what three adhesion molecules do animal cells have

A

tight junction
sealant on ship

desmosome
less adhesion

gap junction
small protein pores that allow ions sugars and small molecules to pas between cells