Cytogenetics

Question 1

Hyperdiploidy revealed by chromosome and FISH analysis is diagnostic for which type of leukemia?

Answer 1

ALL

Question 2

Which is associated with a better prognosis for ALL patients? Hypodiploidy or hyper?

Answer 2

Hyperploidy

Question 3

Which probe in FISH is used to detect ALL?

Answer 3

Centromere Probe study to detect enumeration of chromosomes

Question 4

Which probe in FISH is used to detect AML and associated genes?

Answer 4

Dual fusion or fusion probe—detects translocation

Question 5

What is a diagnostic for AML or APML? How do you treat it?

What is notable in its histology?

Answer 5

• Genotype t(15,17)
• Retanoic acid-
• Auer Rods

Question 6

What is a diagnostic for CML? How do you treat it? What is notable in its histology?

Answer 6

• Genotype t(9,22) -Tyrosine Kinase Inhibitor such as gleevec (if its chronic you are going to be TIRED of SEEING the illness)
• Lobulated large cells

Question 7

How does CMA work?

Answer 7

Single stranded target DNA probes are put on a chip. Patient DNA is then introduced and regions with deletions/duplications can be detected.

Reads are then gathered and interpreted by computers.

Question 8

What do fusion and dual fusion probes test for in FISH?

Answer 8

Translocations

Question 9

What do Break apart probes test for in FISH?

Answer 9

Translocation and rearranged chromosomes

Question 10

What do Whole Chromosome paint probes test for in FISH?

Answer 10

Markers and Translocations