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HEMA311 > Cytochemistry > Flashcards

Cytochemistry Flashcards

1
Q

this is used to differentiate acute
myeloid leukemia from acute
lymphoid leukemia

A

PEROXIDASE
STAIN

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2
Q

In the presence of
hydrogen peroxide, MPO in
granulocytes oxidizes benzidine
dihydrochloride from a colorless to a
reddish brown derivative at the site
of the enzyme

A

PEROXIDASE
STAIN

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3
Q

indication of positive result in peroxidase stain?

A

acute myeloid leukemia

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4
Q

indication of negative result in peroxidase stain

A

acute lymphoid leukemia

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5
Q

stains phospholipids and lipoproteins

A

SUDAN
BLACK B
STAIN

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6
Q

what cell is negative for SBB stain?

A

lymphocytic cell

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7
Q

indication of positive rx of SBB?

A

ACUTE MYELOID LEUKEMIA

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8
Q

indication of negative result of SBB?

A

Acute lymphoid leukemia

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9
Q

what color is the stain of SBB?

A

black or bluis black

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10
Q

It stains lipids, such as
sterols, neutral fats, and
phospholipids because of the
solubility of the dye in lipid particles
and appears dense black.

A

SUDAN
BLACK B
STAIN

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11
Q

differentiate granulocytic from
monocytic leukemias

A

STAIN FOR
ESTERASES

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12
Q

detects esterase
enzyme present in
primary granules of
granulocytic cells
- monocytic cells are
negative in this stain.

A

Naphthol AS-D
Chloroacetate Esterase

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13
Q

indication of positive rxn of Naphthol AS-D
Chloroacetate Esterase?

A

granulocytic leukemia

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14
Q

indication of negative rxn of Naphthol AS-D
Chloroacetate Esterase?

A

monocytic leukemia

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15
Q

gives a positive reaction to
polysaccharides,mucopolysacharid,
glycoproteins, and glycogen

A

PERIODIC
ACID SCHIFF
(PAS)
REACTION

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16
Q

Periodic acid is an
oxidizing agent that converts
hydroxyl groups on adjacent carbon
atoms to aldehydes and further
combined with Schiff’s reagent to
give a red color product

A

PERIODIC
ACID SCHIFF
(PAS)
REACTION

17
Q

postive rxn for periodic acid schiff indicates:

A

Granulocytes Platelet
Erythroleukemia

18
Q

also known as the M6

A

erythroleukemia

19
Q

negative rxn for periodic acid schiff indicate:

A

Acute Lymphoid
Leukemia (ALL), Burkitt Lymphoma

20
Q

Monoclonal or polyclonal
antibodies against factor VIIIrelated antigen have given positive
results in megakaryoblastic
leukemia

A

FACTOR
VIII
ANTIBODIES

21
Q

differentiates CML from a
leukemoid reaction seen in severe
infections

A

LEUKOCYTE
ALKALINE
PHOSPHATASE
(LAP) STAIN

21
Q

differentiates CML from a
leukemoid reaction seen in severe
infections

A

LEUKOCYTE
ALKALINE
PHOSPHATASE
(LAP) STAIN

22
Q

diagnostic tool for confirmation
of hairy cell leukemia

A

ACID
PHOSPHATASE
(TARTRATE
RESISTANT)

23
Q

stain for iron
 it gives a positive reaction in
cases of cells containing iron,
ringed sideroblasts, and
siderocytes

A

PRUSSIAN
BLUE/ PERL’S
STAIN

24
Q

differentiates basophlic
leukemia and mast cell leukemia
from other diseases

A

TOLUIDINE
BLUE

25
Q

most common childhood leukemia,
found in young adults, homogenous
appearance and best prognosis

A

L1 Small lymphoblasts

26
Q

most common in adults, heterogenous
appearance

A

L2 Small and large lymphoblasts

27
Q

leukemic phase of burkitt lymphoma

A

L3 Large homogeneous lymphoblasts

28
Q

m1

A

myeloblasts without maturation

29
Q

m2

A

myeloblasts with maturation

30
Q

m3

A

hypergranular promyelocytic leukemia

31
Q

m3v

A

variant, microgranular promyelocytic leukemia

32
Q

m4

A

myeloblast and monoblast

33
Q

m5

A

monoblast

34
Q

m6

A

erythroleukemia/diguglielmo syndrome

35
Q

m7

A

Megakaryocytic Leukemia

36
Q

characterized by a proliferation of
megakaryoblast and a typical
megakaryocytes in the bone marrow
blood shows pancytopenia

A

Megakaryocytic Leukemia

37
Q

rarest type of the AML

A

Megakaryocytic Leukemia

HEMA311 (5 decks)

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