Cytochemistry Flashcards
this is used to differentiate acute
myeloid leukemia from acute
lymphoid leukemia
PEROXIDASE
STAIN
In the presence of
hydrogen peroxide, MPO in
granulocytes oxidizes benzidine
dihydrochloride from a colorless to a
reddish brown derivative at the site
of the enzyme
PEROXIDASE
STAIN
indication of positive result in peroxidase stain?
acute myeloid leukemia
indication of negative result in peroxidase stain
acute lymphoid leukemia
stains phospholipids and lipoproteins
SUDAN
BLACK B
STAIN
what cell is negative for SBB stain?
lymphocytic cell
indication of positive rx of SBB?
ACUTE MYELOID LEUKEMIA
indication of negative result of SBB?
Acute lymphoid leukemia
what color is the stain of SBB?
black or bluis black
It stains lipids, such as
sterols, neutral fats, and
phospholipids because of the
solubility of the dye in lipid particles
and appears dense black.
SUDAN
BLACK B
STAIN
differentiate granulocytic from
monocytic leukemias
STAIN FOR
ESTERASES
detects esterase
enzyme present in
primary granules of
granulocytic cells
- monocytic cells are
negative in this stain.
Naphthol AS-D
Chloroacetate Esterase
indication of positive rxn of Naphthol AS-D
Chloroacetate Esterase?
granulocytic leukemia
indication of negative rxn of Naphthol AS-D
Chloroacetate Esterase?
monocytic leukemia
gives a positive reaction to
polysaccharides,mucopolysacharid,
glycoproteins, and glycogen
PERIODIC
ACID SCHIFF
(PAS)
REACTION
Periodic acid is an
oxidizing agent that converts
hydroxyl groups on adjacent carbon
atoms to aldehydes and further
combined with Schiff’s reagent to
give a red color product
PERIODIC
ACID SCHIFF
(PAS)
REACTION
postive rxn for periodic acid schiff indicates:
Granulocytes Platelet
Erythroleukemia
also known as the M6
erythroleukemia
negative rxn for periodic acid schiff indicate:
Acute Lymphoid
Leukemia (ALL), Burkitt Lymphoma
Monoclonal or polyclonal
antibodies against factor VIIIrelated antigen have given positive
results in megakaryoblastic
leukemia
FACTOR
VIII
ANTIBODIES
differentiates CML from a
leukemoid reaction seen in severe
infections
LEUKOCYTE
ALKALINE
PHOSPHATASE
(LAP) STAIN
differentiates CML from a
leukemoid reaction seen in severe
infections
LEUKOCYTE
ALKALINE
PHOSPHATASE
(LAP) STAIN
diagnostic tool for confirmation
of hairy cell leukemia
ACID
PHOSPHATASE
(TARTRATE
RESISTANT)
stain for iron
it gives a positive reaction in
cases of cells containing iron,
ringed sideroblasts, and
siderocytes
PRUSSIAN
BLUE/ PERL’S
STAIN
differentiates basophlic
leukemia and mast cell leukemia
from other diseases
TOLUIDINE
BLUE
most common childhood leukemia,
found in young adults, homogenous
appearance and best prognosis
L1 Small lymphoblasts
most common in adults, heterogenous
appearance
L2 Small and large lymphoblasts
leukemic phase of burkitt lymphoma
L3 Large homogeneous lymphoblasts
m1
myeloblasts without maturation
m2
myeloblasts with maturation
m3
hypergranular promyelocytic leukemia
m3v
variant, microgranular promyelocytic leukemia
m4
myeloblast and monoblast
m5
monoblast
m6
erythroleukemia/diguglielmo syndrome
m7
Megakaryocytic Leukemia
characterized by a proliferation of
megakaryoblast and a typical
megakaryocytes in the bone marrow
blood shows pancytopenia
Megakaryocytic Leukemia
rarest type of the AML
Megakaryocytic Leukemia
