Cytochemical Stains Flashcards
Detects myelocytic cels by staining cytoplasmic granular contents
Myeloperoxidase (MPO) & Sudan Black B (SBB)
Detects LYMPHOCYTIC cells and certain abnormal erythrocytic cells by staining glycogen
Periodic acid-Schiff (PAS)
gray-black or red-brown cytoplasmic granules
MPO
Best used for differentiating between AML and ALL
MPO
Stains lipoproteins and phospholipids
SBB
Found in primary and secondary granules of mature and immature neutrophils - negative for lypmhs, megakaryocytes and erythroid precursors
SBB
Useful in separating monocyte precursors from granulocyte precursors
Specific Esterase (SE)
Present in primary granules of neutrophils, but shows negative to weak positive for monos and lymphs
Specific Esterase
Auer rods of AML stain positive with this stain
Specific Esterase
Positive cells show reddish appearance
Nonspecific esterase (NBE)
Used to differentiate myeloid leukemias from monocytic ones
Nonspecific esterase
Positive in monocytic cells and T-cell
NSE
Negative in myeloid cells and megakaryocytes
NSE
_______ makes the nonspecific esterase most specific
Fluoride inhibition step
____ inhibits activity in monocytes, other cells are unaffected
NaFl
positive cells show a brownish appearance
NAE
More sensitive than NBE
NAE
If you add NaFl the reaction goes away in ______
monocytes
stains glyocgen
PAS
Positive in
80% ALL
CLL
Gaucher’s disease
Some AML (AMol, AEL, AMegL)
PAS
Normal RBC precursors are NOT _______
PAS positive
Used to identify T-Lymphocytes (ALL)
Acid Phosphatase (ACP)
ACP _____ in monocytes, neutrophils, and T-cells
Positive
Trap stain positive
Hairy cells
Neutrophils contain this enzyme in their secondary granules
Leukocyte Alkaline Phosphatase (LAP)
Used to help differentiate early CML from other conditions like Leukmoid reaction
LAP
