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Hematology II > Cytochemical Stains > Flashcards

Cytochemical Stains Flashcards

1
Q

Detects myelocytic cels by staining cytoplasmic granular contents

A

Myeloperoxidase (MPO) & Sudan Black B (SBB)

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2
Q

Detects LYMPHOCYTIC cells and certain abnormal erythrocytic cells by staining glycogen

A

Periodic acid-Schiff (PAS)

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3
Q

gray-black or red-brown cytoplasmic granules

A

MPO

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4
Q

Best used for differentiating between AML and ALL

A

MPO

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5
Q

Stains lipoproteins and phospholipids

A

SBB

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6
Q

Found in primary and secondary granules of mature and immature neutrophils - negative for lypmhs, megakaryocytes and erythroid precursors

A

SBB

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7
Q

Useful in separating monocyte precursors from granulocyte precursors

A

Specific Esterase (SE)

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8
Q

Present in primary granules of neutrophils, but shows negative to weak positive for monos and lymphs

A

Specific Esterase

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9
Q

Auer rods of AML stain positive with this stain

A

Specific Esterase

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10
Q

Positive cells show reddish appearance

A

Nonspecific esterase (NBE)

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11
Q

Used to differentiate myeloid leukemias from monocytic ones

A

Nonspecific esterase

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12
Q

Positive in monocytic cells and T-cell

A

NSE

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13
Q

Negative in myeloid cells and megakaryocytes

A

NSE

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14
Q

_______ makes the nonspecific esterase most specific

A

Fluoride inhibition step

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15
Q

____ inhibits activity in monocytes, other cells are unaffected

A

NaFl

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16
Q

positive cells show a brownish appearance

A

NAE

17
Q

More sensitive than NBE

A

NAE

18
Q

If you add NaFl the reaction goes away in ______

A

monocytes

19
Q

stains glyocgen

A

PAS

20
Q

Positive in
80% ALL
CLL
Gaucher’s disease
Some AML (AMol, AEL, AMegL)

A

PAS

21
Q

Normal RBC precursors are NOT _______

A

PAS positive

22
Q

Used to identify T-Lymphocytes (ALL)

A

Acid Phosphatase (ACP)

23
Q

ACP _____ in monocytes, neutrophils, and T-cells

A

Positive

24
Q

Trap stain positive

A

Hairy cells

25
Q

Neutrophils contain this enzyme in their secondary granules

A

Leukocyte Alkaline Phosphatase (LAP)

26
Q

Used to help differentiate early CML from other conditions like Leukmoid reaction

A

LAP

Hematology II (8 decks)

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