Culture Media Preparation (M) Flashcards
True or False.
Diff pathogenic bacteria have same nutritional needs, various types of culture media have been developed for use in diagnostic microbiology
False, because diff pathogenic bacteria have diff nutritional needs
What are fastidious bacteria?
These are bacteria that have relatively complex needs and exceptional media components must be used for growth
What are nonfastidious bacteria?
These are bacteria whereas the nutritional needs of most clinically important bacteria are relatively basic and straightforward
What is the definition of the term culture?
It is a term given to microorganisms that are cultivated in the lab for the purpose of studying them
What is the definition of the term medium?
It is a term given to the combination of ingredients that will support the growth and cultivation of microorganisms by providing all the essential nutrients required for growth (that is, multiplication) in order to cultivate these microorganisms in large #s to study them
How will the medium support the growth and cultivation of microorganisms?
By providing all the essential nutrients required for growth
What are the diff classifications of media based on their physical state?
1) Broth (liquid)
2) Agar (solid)
3) Semi-solid
4) Bi-phasic
What is the composition of broth (in terms of agar)?
No agar is present
What is the composition of agar (in terms of agar)?
0.5 - 1.5 % agar
What is the composition of semi-solid (in terms of agar)?
0.2 - 0.4 % agar
What is the composition of bi-phasic (in terms of agar)?
Both liquid and solid
What is the interpretation for the ff circumstances:
a) Clear broth
b) Turbid broth
a) Indicating no bacterial growth
b) Indicating bacterial growth
What are the classifications based on methods of dispensing?
1) Plated medium
2) Tube Media
What are the components of tube media?
1) Butt
2) Butt / Slant
3) Slant
What are the diff classifications of media (based on their functions)?
1) Enrichment media
2) Supportive media
3) Selective media
4) Differential media
5) Transport media
What is the characteristic of enrichment media?
It contains specific nutrients required for growth of particular bacterial pathogens that may be present alone or w/ other bacterial spp in a pt sx
What is the purpose of enrichment media?
This media type is used to enhance the growth of a particular bacterial pathogen from a mixture of organisms by using nutrient specificity
What are the exs of enrichment media?
1) Choc agar
2) SBA
3) Sel-F
4) Alkaline Peptone water
5) BCYE
What is the characteristic of supportive media and its function?
It contains nutrients that support growth of most nonfastidious organisms w/out giving any particular organism a growth advantage
What is an ex of supportive medium?
Thioglycollate broth
What is the characteristic of selective media and its function?
1) It contains 1 or more agents that are inhibitory to all organisms except those being sought
2) In other words, these media select for the growth of certain bacteria to the disadvantage of others
Does selective media contain inhibitory agents?
Yes
What are the inhibitory agents present in selective media?
1) Dyes
2) Bile salts
3) Alcohols
4) Acids
5) Antibiotics
What are the exs of selective media?
1) DCA
2) LJ medium
3) HE
4) MSA
5) XLD
6) BCYE
What is the characteristic of differential media (or why is this called differential)?
This agar is also differential because the appearance of colonies produced by certain bacterial spp is readily distinguishable
What is the purpose (or action) of differential media (or why is it called differential)?
It employs some factors (or factors) that allows colonies of 1 bacterial spp or type to exhibit certain metabolic or culture characteristics that can be used to distinguish them from other bacteria growing on the same agar plate
What are the exs of differential media?
1) McConkey
2) EMB
3) MSA
What is the purpose of transport media?
These are used to maintain the viability of certain delicate organisms in clinical sxs during their transport to the lab