Culture Media and its Preparation page 6

Question 1

effective short term preservation

Answer 1

refrigeration (4°C)

Question 2

What are the things that can be refrigerated

Answer 2

broth
slants
stabs
streaks

Question 3

cultures are stabbed deeply into agar using a inoculating needle

Answer 3

stab

Question 4

at what temperature must stab cultures be stored?

Answer 4

room or lower temperature

Question 5

cultures may be streaked onto the surface of the solid medium in a slant tube

Answer 5

slant method

Question 6

at what temperature must slant cultures be stored?

Answer 6

room or lower temp

Question 7

freeze-drying of cultures

Answer 7

lyophilization

Question 8

cultures are first frozen and then dried under high vacuum

Answer 8

lyophilization

Question 9

how can freeze-dried cultures be revived?

Answer 9

broth

Question 10

effective long term method of storage

Answer 10

lyophilization and freezing

Question 11

broth cultures are mixed with various ingredients to limit damage

Answer 11

freezing

Question 12

broth cultures are mixed with ____ to limit damage upon freezin and then frozen to temperatures -50°C to -95°C

Answer 12

glycerol, DMSO

Question 13

temperature in freezing

Answer 13

-50°C to -95°C

Question 14

gives important clues as to identify of their constituent microorganisms

Answer 14

colony morphology

Question 15

important classes of characteristics of colony morphology

Answer 15

size
type of margin
colony elevation
colony texture
light transmission
colony pigmentation