Culture Media and its Preparation page 3 Flashcards
microorganisms that grow in a culture medium
culture
to grow microorganisms in a culture medium
to culture
solutions containing all of the nutrients and necessary physical growth parameters necessary for microbial growth
culture media
true or false: all microorganisms can grow in any given culture medium
False;
media can be distinguished qualitatively as:
solid vs broth
non-synthetic vs chemically defined
reducing
selective
differential
media containing agar or some other, mostly inert solidifying agent
solid medium
has physical structure and this allows bacteria to grow physically informative
solid medium
solid medium is usually used as/in
slants
stabs
stab-slant
petri dishes
pile or mass of a sufficiently large number of cells, growing on or in solid medium, that they are visible to the naked eye
colony
media lacking a solidifying matrix
broth medium
different types of chemically undefined medium
(non-synthetic medium)
simple non-synthetic medium
complex non-synthetic medium
contains at least one that is neither purified nor completely characterized nor even completely consistent from batch to batch
non-synthetic medium
operationally defined, is capable of meeting the nutrient requirements of organisms requiring relatively few growth factors
simple non-synthetic medium
supplies more additional types of growth factors
complex non-synthetic medium
consequently able to support the growth of more fastidious microorganisms than can simple non-synthetic medium
complex non-synthetic medium
prepared from purified ingredients and therefore whose exact composition is known
chemically defined synthetic medium
True or False: chemically defined synthetic medium is not trivial for fastidious organisms
true
