Culture Media and its Preparation page 5

Question 1

method of culturing microorganisms in which all of the individuals in a culture have descended from a single individual

Answer 1

pure culture

Question 2

basis of pure culture technique

Answer 2

isolation

Question 3

colony isolation is usually done by culturing methods such as

Answer 3

streaking
pouring
spreading

Question 4

basis of pure culture technique is the isolation, in colonies, of individual cells, and their descendants, from other colonies of individuals

Answer 4

colony isolation

Question 5

isolation procedure is repeated at least once to make sure that an isolated colony is truly derived from only a single cell

Answer 5

isolation from the wild

Question 6

method of melted agar inoculation followed by petri dish incubation

Answer 6

pour plate

Question 7

in pour plating, melted agar must be cooled into

Answer 7

45 degrees celsius

Question 8

because pour plate embeds colonies in agar, it can supply a sufficiently oxygen deficient environment that it can allow the growth and quantification of

Answer 8

microaerophiles

Question 9

liquid cultures are spread on the agar surface using a devise that looks more or less like a hockey stick

Answer 9

spreading a plate

Question 10

preserving cultures is important for

Answer 10

scientific reasons
identification
vaccine production
industrial use

Question 11

methods of preserving culture include

Answer 11

refrigeration
stabs
slants
lyophilization
freezing