Culture Media

Question 1

A semi-solid, non nutritive media with sodium thiosulphate to prevent oxidative changes to the organism

Maintains organism viability but does not support growth

Answer 1

Stuart’s Medium

Question 2

Used for the collection of stool specimen for bacteriology studies

Answer 2

Buffered glycerol saline

Question 3

used for the collection of stool for parastitology studies

Answer 3

5% Formalin

Question 4

Chocolate tellurite is a selective medium, what is it used to culture ?

Answer 4

Corynebacterium species

Question 5

This media contains the basic nutrients needed to provide growth of most organism

Answer 5

Nutrient Broth/Meat broth

Question 6

Nutrient broth to which 2% agar has been added

Answer 6

Nutrient Agar

Question 7

Heated sheep or horse blood to release extra nutients from the blood

Factor X and V

Answer 7

Chocolate Agar

Question 8

This differentiates lactose fermenters from non-lactose fermenters and can be used to selective grow gram negative bacilli (enterobacteriacea) and inhibit gram positive cocci

Answer 8

MacConkey’s Agar

Question 9

This selective medium is heated blood agar with CVNB (colistin, vancomycin, nystatin, bactrim) and isolates Neisseria Gonorrhea from genital swabs as it inhibits the growth of the normal flora

Answer 9

Thayer Martin agar

Question 10

This medium is used to identify Salmonella, separate from Shigella species.
Salmonella will form yellow colonies which then turn pink with black centres

Shigella does not ferment the xylose so the colonies remain pink, and it also does not oxidize the thiosulphate to H2S so no black dots form

Answer 10

XLD
Xylose, lysine, deoxycholate Agar

Question 11

Used for the isolation of bordetella pertussis

Answer 11

Bordet Gengou Agar

Question 12

Used for the isolation of Corynebacterium diphtheria

Potassium tellurite is added, and is reduced by C.diptheria forming black colonies

Answer 12

tinsdale Agar/ Chocolate tellurite Agar

Question 13

Used to demonstrate metachromatic granules of corynebacterium diptheria

Answer 13

Loeffler’s Serum Slope

Question 14

This medium is used to isolate mycobacterium (M.tuberculosis)

It contains egg and an inhibitory green pigment dye to gram pos and gram neg bacteria

Answer 14

Lowenstein-Jenson Medium

Question 15

This media suppresses coliform bacilli from faeces and enhances the growth of enteric pathogens

Answer 15

Selenite/Gram negative broth

Question 16

These media are used for the cultivation of Leptospira

Answer 16

Fletcher’s medium, Ellinghausen Medium

Question 17

This medium has low pH, high sugar and inhibits bacterial growth

Ideal for fungi growth

Answer 17

Sabouraud’s Dextrose

Question 19

What is the name of the specific culture medium used to grow Candida Albicans specifically

Answer 19

Cornmeal Agar