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Culture Media Flashcards

1
Q

What are the steps to culture media dispensing/distribution for plated media

A

Weigh the lyophilized powder
Dissolution
Autoclave
Dispense

Note: Almost same with the tube media. Difference: Autoclave & Dispense

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2
Q

What are the steps to culture media dispensing/distribution for tube media

A

Weigh the lyophilized agar
Dissolution
Dispense
Autoclave

Note: Almost same with the tube media. Difference: Autoclave & Dispense

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3
Q

Nutrient Agar & Trypticase Soy Broth are examples of what type of media

Classification of media according to fxn

A

Simple/Supportive/General Purpose

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4
Q

Give 2 examples of the most common enriched media used in the lab

A

BAP
CAP

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5
Q

LIM’s Broth, Tetrathionate, Selenite F, Alkaline Peptone water are examples of

Classification of media according to fxn

A

Enrichment media

Enriched media: Solid + additional supplements
Enrichment media: Liquid w/out additional supplements

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6
Q

Crystal violet, Gentian violet, Bile salt, Carbolfuchsin are inhibitors of what type of organisms

(Gram positive, Gram Negative)

A

Gram positive

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7
Q

Potassium Tellurite, Sodium Azide are inhibitors of what type of organisms

(Gram pos or Gram Neg)

A

Gram negative

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8
Q

What is used to inhibit swarming of certain organisms?

A

Alcohol, Chloral Hydrate, Trimethoprim lactate

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9
Q

Phenyl/Ethyl Alcohol, Columbia Agar, Colistin nalidixic agar are selective culture media for?

A

Gram Positive organisms

Inhibits gram negative organisms

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10
Q

This is a selective media for M. tuberculosis

A

Lowenstein Jensen

A protein enriched media

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11
Q

What is the inhibitor used for Lowenstein Jensen Agar when cultivating M. tuberculosis

A

Malachite green

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12
Q

Selective media for Corynebacterium diphtheriae

A

Loeffler’s medium

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13
Q

Inhibitor used in Loeffler’s medium when cultivating Corynebacterium diphtheriae

A

Potassium Tellurite

*Inhbitor for gram negative bacteria

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14
Q

Selective medium for N. gonorrhoea

A

Thayer Martin, Modified Thayer Martin, Martin Lewis, New York City Agar, GC-LECT

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15
Q

Antibiotics used for inhibition in Thayer Martin Agar & Modified Thayer Martin Agar

A

Vancomysin
Colistin
Nystatin

Modified Thayer Martin: VCN antibiotics + Trimethoprin Lactate (Inhibits swarming)

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16
Q

Antibiotics used for inhibition in Martin Lewis

A

Vancomysin
Colistin
Anisomysin (Antifungal)
Trimethoprim

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17
Q

Antibiotics used for inhibition in New York City Agar

A

Vancomysin
Colistin
Amphotericin B (Antifungal)
Nystatin?
Trimethoprim

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18
Q

Antibiotics used for inhibition in GC-LECT

A

Vancomysin
Colistin
Anisomycin
Trimethoprim
Lincomysin (Gram neg inhibition)

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19
Q

Selective medium for Staphylococcus

A

Mannitol Salt Agar

Inhibitor: 7.5% NaCl
pH Indicator: Phenol Red
CHO: Mannitol

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20
Q

Selective differential for Vibrio

A

Thiosulfate Bile Sucrose Agar

CHO: Sucrose
pH Indicator: Bromthymol Blue

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21
Q

K. pneumoniae is what type of lactose fermenter

(Rapid, Late, or Non)

A

Rapid Lactose Fermenter

*EKE (E. coli, Klebsiella, Enterobacter)

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22
Q

Erwinia is what type of Lactose Fermenter

(Rapid, Late, or Non)

A

Late Lactose Fermenter

*Yana Eats Half Sugar Cookies
Yersinia, Erwinia, Hafnia, Salmonella arizonae, Shigella sonnei, Serratia, Citrobacter

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23
Q

Serratia is what type of Lactose Fermenter

(Rapid, Late, or Non)

A

Late Lactose Fermenter

*Yana Eats Half Sugar Cookies
Yersinia, Erwinia, Hafnia, Salmonella arizonae, Shigella sonnei, Serratia, Citrobacter

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24
Q

All Salmonella are Non-Lactose fermenter except

A

Salmonella arizonae

*Yana Eats Half Sugar Cookies (Mnemonic for Late-Lactose Fermenter)
Yersinia, Erwinia, Hafnia, Salmonella arizonae, Shigella sonnei, Serratia, Citrobacter

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25
Q

Kluyvera & Cedecea are what type of Lactose fermenter

(Rapid, Late, Non)

A

Non-Lactose Fermenter

*Sugar Can Kill My PaPa Ed
Salmonella, Shigella, Cedecea, Kluyvera, Morganella, Proteus, Providencia, Edwardsiella

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26
Q

What does Lactose fermenters appear as on Eosin Methylene Blue Agar

A

Pink/Purple Colonies

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27
Q

MacConkey Agar is an example of a selective medium for?

A

Gram Negative Enteric Bacilli

*My X Eats His Shit
MacConkey, XLD, Eosin Methylene Blue, HEA, Salmonella Shigella

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28
Q

This organism’s colony has a greenish metallic sheen on EMB agar

A

E. coli

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29
Q

Klebsiella appears _______ on EMB agar

A

Pink mucoidal colonies

*Klebsiella capsule Forming (Some Killers Have Pretty Nice Capsules)
Rapid Lactos Fermenter (EKE)

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30
Q

Enterobacter colonies appear _________ on EMB

A

Pink colonies w/ black centers

Enterobacter are NOT H2S +

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31
Q

What are the inhibitors used in MacConkey Agar

A

Bile Salt & Crystal Violet

Inhibits Gram positive: Bile Salt, Crystal Violet, Gentian Violet, Carbolfuchsin

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32
Q

What inhibitors are present in HEA agar

A

Bile Salt

Inhibits Gram positive: Bile Salt, Crystal Violet, Gentian Violet, Carbolfuchsin

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33
Q

Sugars used in EMB

A

Levine: Lactose
Hot Harris: Sucrose

*LeLa loves eating w/ Hot Sauce

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34
Q

Sugars used in MacConkey Agar

A

Lactose

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35
Q

Sugars used in HEA

A

Lactose, Salicin, Sucrose

*LSS sa Her

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36
Q

What is the H2S indicator used in Salmonella Shigella Agar

A

Ferric Ammonium Citrate

All H2S Media uses FAC as indicator EXCEPT FOR TSI which uses Ferric ammonium sulfide

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37
Q

Salmonella colonies appear as _____ on Salmonella shigella agar

A

Yellow w/ black centers

*Salmonella non-lactose fermenter (Sugar Can Kill My PaPa Ed)
H2S + (SPACE)
*Salmonella Shigella Agar MS Neutral (MacConkey, Salmonella Shigella Agar)

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38
Q

Salmonella arizonae appear as ____ on Salmonella Shigella Agar

A

Red w/ black centers

*Salmonella Arizonae late lactose fermenter (Yana Eats Half Sugar Cookies)
H2S + (SPACE)

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39
Q

Sugars used in XLD

A

Xylose, Lactose, Sucrose

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40
Q

Alkaline Peptone water is a special media used for

A

Vibrio cholerae

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41
Q

Monur’s tellurite taurocholate gelatin agar is a special media for

A

Vibrio cholerae

42
Q

Venkatraman Ramakrishnan is a special media for

A

Vibrio cholerae

43
Q

Elner’s medium is a special media for

A

Clostridium

44
Q

Medium of Duncan & Strong is a special media for

A

Clostridium

45
Q

Medium of Phillips is a special media for

A

Clostridium

46
Q

Alkaline Egg medium is a special medium for

A

Clostridium

47
Q

Fildes blood digest agar is a special media for

A

Haemophilus

*haemoPHI = FIldes

48
Q

Firm agar is a special media for

A

Proteus & C. tetani

*Swarming organisms

49
Q

Glycerol saline transport medium is a special media for

A

Salmonella

50
Q

Hoyle’s tellurite lysed blood agar is a special media for

A

Corynebacterium

51
Q

Wilson & Blair’s Brilliant green bismuth sulphite agar is a special media for

A

Salmonella & Shigella

52
Q

Phenol-Red Egg yolk polymyxin agar is a special media for

A

Bacillus Cereus

53
Q

Pike’s media is a special media for

A

S. pneumoniae, H. influenzae

54
Q

Polymyxi B-lysoxyme-EDTA Thallous Acetate is a special media for

A

B. anthracis

55
Q

Proteose Peptone Yeast Extraction broth is a special media for

56
Q

Salt cooked Meat Broth is a special media for

57
Q

Smith-Noguchi Medium is a special media for

58
Q

Carrot broth is a special media for

A

Group B Strep

59
Q

Wilkins-Chalgren Agar is a special media used as an Antimicrobial susceptibility test for

60
Q

Kouga Agar is a special media for

A

Strongyloides stercoralis

61
Q

Transgrow medium is an example of what type of media

A

Transport Media

Together with Jembec, Stuart & Amie, Cary Blair, Venkatraman Ramakrishnan

62
Q

Agar depth for Mueller Hinton Agar

63
Q

pH for Mueller Hinton Agar

64
Q

Used in the differentiation of non-fermentative gram-negative bacteria, particularly Pseudomonas aeruginosa

A

Acetamide Agar

65
Q

Enrichment medium used for cultivation of vibrio species from feces

A

Alkaline Peptone Water

66
Q

Differential Isolation and presumptive identification of Group D streptococci and enterococci

A

Bile Esculin Agar

67
Q

Cultivation of non-fastidious microorganisms, used for the determination of hemolytic reactions

A

Blood Agar Plate

68
Q

Isolation of Bordatella pertussis and Bordatella parapertussis

A

Bordet-Gengou Agar

69
Q

Enrichment for Legionella spp.
Supports growth of Francisella and Nocardia spp

A

Buffered Charcoal-Yeast Extract Agar (BCYE)

70
Q

Used to differentiate Enterococci from other Streptococci

A

Bile Esculin Agar

71
Q

Highly selective medium used for isolation of Salmonella Spp

A

Bismuth Sulfide Agar

72
Q

Selective medium for Campylobacter spp

A

Campy-blood agar

73
Q

Selective holding medium for recovery of Campylobacter spp

A

Campylobacter thioglycolate broth

74
Q

Selective for Yersinia spp. maybe useful for isolation of Aeromonas spp

A

Cefsulodin-Irgasan-Novobiocin medium (CIN)

75
Q

Selective isolaton of gram positive cocci in heavily contaminated sample (stool)

A

Columbia Colistin-Nalidixic Acid Agar (CNA)

76
Q

For isolation of Corynebacterium diphtheriae

A

Cysteine tellurite blood agar

77
Q

Used for isolation and differentiation of Staphylococci based on mannitol fermentation and gelatinase activity

A

Chapman stone medium

78
Q

Cultivation of Francisella tularensis

A

Cystine Heart Agar

79
Q

Detection of coliform bacteria in water

A

EC medium

aka E. coli broth

80
Q

Used for cultivating Streptococci & Lactobacilli, particularly in dairy procedures

A

Elliker Broth

81
Q

Enrichment medium for Leptospira

A

Fletcher’s medium

82
Q

Differential selective medium for the isolation and differentiation of Salmonella and Shigella spp from other gram negative bacilli

A

Hektoen Enteric Agar

83
Q

Selective and differential medium for Gardnerella vaginalis

A

Human Blood Tween Bilayer

84
Q

Isolation and growth of Corynebacterium spp

A

Loeffler’s medium

85
Q

Isolation and differential of lactose fermenting and non-lactose fermenting gram negative bacilli

A

MacConkey Agar

86
Q

For the isolation and differentiation of E. coli 0157:H7 in stool specimens

A

MacConkey Sorbitol Agar

87
Q

Selective differentiation of Staphylococci

A

Mannitol Salt Agar

88
Q

Recommended for isolation of Lactobacillus spp.

A

MRS (deMan, Rogosa, & Sharpe)

89
Q

Recommended for antimicrobial disc diffusion susceptibility testing

A

Mueller Hinton Agar (MHA)

90
Q

Cultivation of bacteria & enumeration of organisms

A

Nutrient Agar

91
Q

Selective for Neisseria gonorrhoeae

A

New York City Agar

92
Q

Qualitative procedures for the isolation of Mycobacteria

A

Petragnani Medium

93
Q

Enrichment and selective medium for isolation of Bordatella pertussis

A

Regan Lowe

94
Q

Selective for Salmonella some Shigella spp

A

Salmonella-Shigella spp

95
Q

Selective for Campylobacter & Helicobacter spp

A

Schaedler agar

96
Q

Enrichment of isolation of Salmonella spp

A

Selenite agar

97
Q

Selective for Campylobacter spp

A

Skirrow agar

98
Q

Selective for N. gonorrhoeae & N. meningitidis

A

Thayer-Martin Agar

99
Q

Selective and differential for Vibrio spp

A

Thiosulfate Citrate Bile Salt Agar

100
Q

Selective & enrichment for Streptococcus agalactiae in female genital specimens

A

Todd-Hewitt broth

101
Q

Enrichment broth used for sub-culturing various bacteria from primary agar plates

A

Trypticase Soy Broth

102
Q

Isolation and differentiation of Salmonella and Shigella spp. from other gram negative enteric bacilli

A

Xylose Lysine Deoxycholate Agar

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